Laboratoire d'Ingenierie pour les Systemes Complexes, Cemagref, Campus Universitaire des Cezeaux, France.
Biofouling. 2009 Nov;25(8):695-703. doi: 10.1080/08927010903104984.
A digital image correlation (DIC) method was applied to characterize the mechanical behavior of Pseudomonas aeruginosa biofilms in response to wall shear stress using digital video micrographs taken from biofilm flow cells. The appearance of the biofilm in the transmitted light photomicrographs presented a natural texture which was highly conducive to random encoding for DIC. The displacement fields were calculated for two biofilm specimens. The DIC method concurred with previous analysis showing that biofilms exhibit viscoelastic behavior, but had the advantage over simple length measurements of longitudinal strain that it could precisely measure local strains in length (x) and width (y) within biofilm clusters with a 2 mum resolution as a function of time and wall shear stress. It was concluded that DIC was more accurate at measuring elastic moduli than simple length measurements, but that time-lapse 3D images would enable even more accurate estimates to be performed.
采用数字图像相关（DIC）方法，通过对取自生物膜流动池的数字视频显微照片进行分析，来描述铜绿假单胞菌生物膜对壁面剪应力的力学响应行为。生物膜在透射光显微照片中的外观呈现出一种自然纹理，非常有利于 DIC 的随机编码。对两个生物膜样本进行了位移场计算。DIC 方法与先前的分析结果一致，表明生物膜表现出粘弹性行为，但与简单的长度测量相比，它具有纵向应变的优势，因为它可以精确测量生物膜簇中长度（x）和宽度（y）的局部应变，分辨率为 2 微米，作为时间和壁面剪应力的函数。研究结论认为，DIC 在测量弹性模量方面比简单的长度测量更准确，但延时 3D 图像将能够进行更准确的估计。
