Wanless C, Barker S, Puddefoot J R, Panahy C, Goode A W, Vinson G P, Phillips I R
Department of Biochemistry, Queen Mary and Westfield College, University of London, U.K.
Anticancer Res. 1991 Jan-Feb;11(1):139-42.
Using a fragment of the estrogen receptor (ER) cDNA as a probe, Southern blots of HindIII-digested genomic DNA, from breast tumours and non-cancerous tissue, revealed a 1.6 kb hybridizing fragment (1.6KbHF) in 3 out of 30 tumours, and in 31 out of 80 mastectomy samples (16 sectors from each of 5 patients), which was significantly correlated with the absence of progesterone receptor. In 4 of the patients, sectors which showed the 1.6KbHF co-existed in the same breast with sectors which did not. Neither the use of higher concentrations of the restriction enzyme, not high salt extraction of DNA changed the abundance of the 1.6KbHF relative to other bands. Similar studies on EcoRI digests of the same DNA samples failed to show any variants. As a further control, hybridization of the same Southern blots of HindIII-digested DNA, with a cytochrome P450IIA3 cDNA, also failed to show variants in these samples. It is concluded that the presence of the 1.6KbHF represents a somatic change in the nucleus of these samples which may have functional importance.
以雌激素受体（ER）cDNA片段作为探针，对来自乳腺肿瘤和非癌组织的经HindIII消化的基因组DNA进行Southern杂交分析，结果显示，在30个肿瘤样本中有3个、80个乳房切除术样本（来自5名患者，每人16个切片）中有31个出现了一条1.6 kb的杂交片段（1.6KbHF），该片段与孕激素受体缺失显著相关。在4名患者中，显示有1.6KbHF的切片与同一乳房中未显示该片段的切片并存。无论是使用更高浓度的限制性内切酶，还是采用高盐法提取DNA，相对于其他条带，1.6KbHF的丰度均未改变。对相同DNA样本进行EcoRI消化后的类似研究未显示任何变异条带。作为进一步的对照，用细胞色素P450IIA3 cDNA对经HindIII消化的DNA的相同Southern杂交膜进行杂交，这些样本中也未显示变异条带。得出的结论是，1.6KbHF的存在代表了这些样本细胞核中的一种体细胞变化，可能具有功能重要性。
