Department of Civil Engineering & Construction, Bradley University, Peoria, Illinois, USA.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2009 Nov;44(13):1315-26. doi: 10.1080/10934520903212956.
The biodegradation of naphthalene, phenanthrene, and pyrene were studied in batch reactors using an anaerobic acetate-fed enrichment culture developed from the Greater Peoria Sanitary District Wastewater Treatment Facility. Baseline kinetic experiments showed that maximum specific substrate utilization for the acetate-fed enrichment culture was 2.72 mg acetate/mg cell/day and the endogenous decay coefficient was 0.043 day(-1). Maximum specific substrate utilization rates in mg substrate/mg cell/day for naphthalene, phenanthrene and pyrene were 0.57, 0.009 and 0.007, respectively. The half saturation constant K(S) in mg/L were 0.075, 0.01 and less than 0.01, respectively. The endogenous decay coefficient (in units of day(-1)) in the presence of naphthalene, phenanthrene and pyrene were 0.06, 0.07 and 0.04, respectively. Results from this study indicated that naphthalene and phenanthrene were more easily biodegradable than pyrene. These results suggest that technologies using anaerobic biodegradation using a relatively simple enrichment culture from a wastewater treatment plant may be successful in treating PAH contaminated wastes.
采用从大皮奥里亚污水处理厂开发的厌氧乙酸喂养富集培养物，在批量反应器中研究了萘、菲和芘的生物降解。基线动力学实验表明，乙酸喂养富集培养物的最大比基质利用速率为 2.72mg 乙酸/mg 细胞/天，内源性衰减系数为 0.043 天（-1）。萘、菲和芘的最大比基质利用速率（mg 基质/mg 细胞/天）分别为 0.57、0.009 和 0.007。在 mg/L 存在时的半饱和常数 K(S)分别为 0.075、0.01 和小于 0.01。萘、菲和芘存在时的内源性衰减系数（单位为天（-1））分别为 0.06、0.07 和 0.04。本研究结果表明，萘和菲比芘更容易生物降解。这些结果表明，使用来自污水处理厂的相对简单的厌氧生物降解富集培养物的技术可能成功地用于处理多环芳烃污染的废物。
