Prakash Thazha P, Naik Nishant, Sioufi Namir, Bhat Balkrishen, Swayze Eric E
Department of Medicinal Chemistry, Isis Pharmaceuticals Inc., Carlsbad, California 92008, USA.
Nucleosides Nucleotides Nucleic Acids. 2009 Oct;28(10):902-10. doi: 10.1080/15257770903316145.
In a search to identify chemical modifications to improve the properties of siRNA, we have investigated the effect of the 2 '-O-methyl-2-thiouridine modification on the biological activity of siRNA. Our results indicate that judicious placement of 2 '-O-methyl-2-thiouridine residues could lead to modified siRNA with activity in mammalian cells.
在寻找能够改善小干扰RNA（siRNA）特性的化学修饰方法的过程中，我们研究了2'-O-甲基-2-硫代尿苷修饰对siRNA生物活性的影响。我们的结果表明，合理引入2'-O-甲基-2-硫代尿苷残基能够产生在哺乳动物细胞中具有活性的修饰后的siRNA。
