Department of Chemistry, Zhejiang University, Hangzhou, PR China.
J Sep Sci. 2010 Apr;33(8):1018-23. doi: 10.1002/jssc.200900588.
A practical approach for rapidly screening antioxidants against superoxide anion radicals from complex mixtures was developed based on the quantitative difference in active compounds before and after their reaction. To test the effectiveness of the approach, seven flavonoids with antioxidative properties were investigated both individually and in a mixture. Using the approach, antioxidants could be rapidly separated and screened with a ranked order of activities in the meantime. The radical scavenging activities were in the following order: quercetin > kaempferol > fisetin > puerarin > luteolin > rutin > baicalein. The order of activity was conducted by comparing the scavenging ratio of the antioxidant, which was completely consistent with the results obtained from the traditional electronic spin resonance. Then, the method was successfully applied to black tea extracts. This approach is fast and convenient for screening, isolating, and analyzing potential antioxidants from a mixture with good quantitative and reproducible ability.
建立了一种从复杂混合物中快速筛选超氧阴离子自由基抗氧化剂的实用方法，该方法基于反应前后活性化合物的定量差异。为了测试该方法的有效性，分别对七种具有抗氧化特性的类黄酮进行了单独和混合物的研究。使用该方法，同时可以快速分离和筛选具有活性的抗氧化剂，并对其活性进行排序。自由基清除活性的顺序为：槲皮素>山柰酚>非瑟酮>葛根素>木樨草素>芦丁>黄芩素。通过比较抗氧化剂的清除率来确定活性顺序，这与传统的电子自旋共振实验结果完全一致。然后，该方法成功应用于红茶提取物。该方法快速、方便，可用于从混合物中筛选、分离和分析潜在的抗氧化剂，具有良好的定量和重现性。
