Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, UP, India.
Environ Technol. 2009 Dec 14;30(14):1519-27. doi: 10.1080/09593330903246432.
Salt-fractionated bitter gourd (Momordica charantia) proteins were employed for the decolorization of disperse dyes in the presence of H2O2. The effect of various experimental conditions such as concentration of enzyme, H2O2, phenol, reaction time, pH and temperature on the decolorization of dyes was investigated. Dyes were recalcitrant to the decolorization catalysed by bitter gourd peroxidase. However, these dyes were decolorized significantly in the presence of a redox mediator, phenol. Bitter gourd peroxidase (0.215 U/mL) could decolorize about 60% of Disperse Red 17 in the presence of 0.2 mM phenol, whereas Disperse Brown 1 was decolorized by only 40% even in the presence of 0.4 mM phenol. Maximum decolorization of dyes was achieved in the presence of 0.75 mM H2O2 in a buffer ofpH 3.0 and 40 degrees C within 30 min. The K(m) values obtained were 0.625 mg/(L x h) and 2.5 mg/(L x h) for Disperse Red 17 and Disperse Brown 1, respectively. In all the experiments, Disperse Brown 1 was found to be more recalcitrant to decolorization catalysed by bitter gourd peroxidise, as compared to Disperse Red 17.
采用盐析苦瓜（Momordica charantia）蛋白在 H2O2 存在下对分散染料进行脱色。考察了酶浓度、H2O2、苯酚、反应时间、pH 和温度等各种实验条件对染料脱色的影响。染料对苦瓜过氧化物酶催化的脱色具有抗药性。然而，在氧化还原介体苯酚的存在下，这些染料显著地被脱色了。苦瓜过氧化物酶（0.215 U/mL）在 0.2 mM 苯酚存在下可使分散红 17 脱色约 60%，而即使在 0.4 mM 苯酚存在下，分散棕 1 也仅被脱色 40%。在 pH 3.0 和 40°C 的缓冲液中，在 30 分钟内加入 0.75 mM H2O2 可实现最大的染料脱色。分别获得了 0.625 mg/（L·h）和 2.5 mg/（L·h）的Disperse Red 17 和 Disperse Brown 1 的 K(m)值。在所有实验中，与 Disperse Red 17 相比，Disperse Brown 1 被发现对苦瓜过氧化物酶催化的脱色更具抗药性。
