Matto Mahreen, Husain Qayyum
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India.
J Hazard Mater. 2009 May 30;164(2-3):1540-6. doi: 10.1016/j.jhazmat.2008.09.069. Epub 2008 Sep 26.
Bitter gourd peroxidase immobilized on the surface of concanavalin A layered calcium alginate-starch beads was used for the successful and effective decolorization of textile industrial effluent. Effluent was recalcitrant to the action of bitter gourd peroxidase; however, in the presence of some redox mediators, it was successfully decolorized. Effluent decolorization was maximum (70%) in the presence of 1.0mM 1-hydroxybenzotriazole within 1h of incubation. However, immobilized bitter gourd peroxidase showed maximum decolorization at pH 5.0 and 40 degrees C. Immobilized bitter gourd peroxidase decolorized more than 90% effluent after 3h of incubation in a batch process. The two-reactor system, one reactor containing immobilized peroxidase and the other had activated silica, was quite effective in the decolorization of textile effluent. The system was capable of decolorizing 40% effluent even after 2 months of continuous operation. The absorption spectra of the untreated and treated effluent exhibited a marked difference in absorbance at various wavelengths. Immobilized peroxidase/1-hydroxybenzotriazole system could be employed for the treatment of a large volume of effluent in a continuous reactor.
固定在伴刀豆球蛋白A层状海藻酸钙-淀粉珠表面的苦瓜过氧化物酶被用于成功且有效地使纺织工业废水脱色。该废水对苦瓜过氧化物酶的作用具有抗性；然而，在一些氧化还原介质存在的情况下，它成功实现了脱色。在1.0 mM 1-羟基苯并三唑存在下，孵育1小时后废水脱色率最高（70%）。然而，固定化苦瓜过氧化物酶在pH 5.0和40℃时表现出最大脱色效果。在分批处理中，孵育3小时后，固定化苦瓜过氧化物酶使超过90%的废水脱色。双反应器系统，一个反应器含有固定化过氧化物酶，另一个含有活化硅胶，在纺织废水脱色方面相当有效。即使连续运行2个月后，该系统仍能够使40%的废水脱色。未处理和处理后废水的吸收光谱在不同波长下的吸光度表现出明显差异。固定化过氧化物酶/1-羟基苯并三唑系统可用于在连续反应器中处理大量废水。
