Alipieva Kalina, Petreska Jasmina, Gil-Izquierdo Angel, Stefova Marina, Evstatieva Ljuba, Bankova Vassya
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 9, 1113 Sofia, Bulgaria.
Nat Prod Commun. 2010 Jan;5(1):51-4.
The influence of the extraction method on the yield and composition of extracts of Sideritis (Pirin mountain tea) has been studied. Maceration, ultrasound-assisted (USAE) and microwave assisted extraction (MAE) were applied. Total phenolics and total flavonoids were quantified spectrophotometrically, and individual compounds were analyzed by HPLC-DAD-MS(n). This preliminary study reveals that the traditional way of tea preparation from Sideritis is the most appropriate in order to extract the maximum of total flavonoids and total phenolics. In the case of methanol extraction, the optimal method is USAE.
研究了提取方法对希德鸢尾（皮林山茶）提取物得率和成分的影响。采用了浸渍法、超声辅助提取法（USAE）和微波辅助提取法（MAE）。通过分光光度法定量测定总酚和总黄酮，并采用高效液相色谱-二极管阵列-质谱联用仪（HPLC-DAD-MS(n)）分析单个化合物。这项初步研究表明，为了最大程度地提取总黄酮和总酚，传统的希德鸢尾茶制备方法是最合适的。在甲醇提取的情况下，最佳方法是超声辅助提取法。
