Pronina T S, Kalas A, Ugriumov M V
Ontogenez. 2010 Jan-Feb;41(1):41-6.
The effect of serotonin on the formation of neurons producing gonadotropin-releasing hormone (GnRH) during embryogenesis of Wistar rats was studied. The neurons producing GnRH were detected immunocytochemically on days 18 and 21 of embryonic development and on day 15 of postnatal development of rats with normal serotonin metabolism and rats in which the synthesis of serotonin was inhibited by p-chlorophenylalanine. The total number of GnRH neurons in serotonin deficiency was larger than in the case of its normal metabolism at all developmental stages studied. This is an indirect evidence for the inhibitory effect of serotonin on the formation ofGnRH neurons. To confirm the morphogenetic effect of serotonin, we studied the rate of formation of GnRH neurons by injecting bromodeoxyuridine in the formation period of these neurons. It was found that serotonin deficiency had no effect on the time of formation of GnRH neurons: over 97% of neurons formed on days 11 to 15 of embryonic development both in the experimental and control groups. Note that, in serotonin deficiency, the maximum number of GnRH neurons formed one day later than in the normal state. Thus, serotonin inhibits the proliferation of GnRH neuron progenitor cells and thereby has a morphogenetic effect on the development of these neurons.
研究了血清素对Wistar大鼠胚胎发育过程中产生促性腺激素释放激素（GnRH）的神经元形成的影响。在胚胎发育的第18天和第21天以及出生后第15天，通过免疫细胞化学方法检测了血清素代谢正常的大鼠和血清素合成被对氯苯丙氨酸抑制的大鼠中产生GnRH的神经元。在所研究的所有发育阶段，血清素缺乏时GnRH神经元的总数均多于其正常代谢时的情况。这是血清素对GnRH神经元形成具有抑制作用的间接证据。为了证实血清素的形态发生作用，我们在这些神经元的形成期注射溴脱氧尿苷，研究了GnRH神经元的形成速率。结果发现，血清素缺乏对GnRH神经元的形成时间没有影响：实验组和对照组中超过97%的神经元在胚胎发育的第11至15天形成。需要注意的是，在血清素缺乏时，GnRH神经元的最大数量比正常状态下晚一天形成。因此，血清素抑制GnRH神经元祖细胞的增殖，从而对这些神经元的发育产生形态发生作用。
