来自一名囊性纤维化患者呼吸道粘蛋白的两种硫酸化寡糖的结构。快原子轰击质谱和1H核磁共振光谱研究。
Structure of two sulphated oligosaccharides from respiratory mucins of a patient suffering from cystic fibrosis. A fast-atom-bombardment m.s. and 1H-n.m.r. spectroscopic study.
作者信息
Lamblin G, Rahmoune H, Wieruszeski J M, Lhermitte M, Strecker G, Roussel P
机构信息
Unité I.N.S.E.R.M. no 16 and G.D.R. C.N.R.S. no 15, Lille, France.
出版信息
Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):199-206. doi: 10.1042/bj2750199.
Abstract
Human respiratory-mucin glycopeptides were isolated from the sputum of a patient suffering from cystic fibrosis. They were subjected to treatment with alkaline borohydride. Application of ion-exchange chromatography afforded carbohydrate fractions containing sulphate. One of these fractions containing a mixture of sulphated oligosaccharides was subsequently submitted to gel-filtration chromatography and h.p.l.c. Two sulphated oligosaccharide-alditols, A and B, were prepared; their structure was determined by means of 400 MHz 1H-n.m.r. spectroscopy and fast-atom-bombardment m.s. They had a core type 2 and the sulphate was 3-linked to a terminal galactose residue: (Formula; see text)
摘要
从一名囊性纤维化患者的痰液中分离出了人呼吸道粘蛋白糖肽。将它们用碱性硼氢化物处理。通过离子交换色谱法得到了含硫酸盐的碳水化合物馏分。随后，将其中一个含有硫酸化寡糖混合物的馏分进行凝胶过滤色谱法和高效液相色谱法分析。制备了两种硫酸化寡糖糖醇A和B；它们的结构通过400兆赫的1H-核磁共振光谱法和快原子轰击质谱法确定。它们具有核心2型结构，硫酸盐与末端半乳糖残基以3-连接：（分子式；见正文）
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本文引用的文献
1
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J Biol Chem. 1982 May 10;257(9):4709-18.
2
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Arch Oral Biol. 1981;26(4):315-7. doi: 10.1016/0003-9969(81)90053-4.
4
Complex structure of human bronchial mucus glycoprotein.人支气管黏液糖蛋白的复杂结构
Eur J Biochem. 1984 Jul 16;142(2):209-18. doi: 10.1111/j.1432-1033.1984.tb08273.x.
5
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6
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7
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8
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