源自囊性纤维化患者支气管黏液糖蛋白的五种唾液酸化寡糖的一级结构测定。通过500兆赫1H核磁共振光谱揭示的NeuAcα(2→3)Galβ(1→4)[Fucα(1→3)]GlcNAcβ(1→.)结构元件的存在情况。
Primary structure determination of five sialylated oligosaccharides derived from bronchial mucus glycoproteins of patients suffering from cystic fibrosis. The occurrence of the NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)] GlcNAc beta(1----.) structural element revealed by 500-MHz 1H NMR spectroscopy.
作者信息
Lamblin G, Boersma A, Klein A, Roussel P, van Halbeek H, Vliegenthart J F
出版信息
J Biol Chem. 1984 Jul 25;259(14):9051-8.
The structure of sialylated carbohydrate units of bronchial mucins obtained from cystic fibrosis patients was investigated by 500-MHz 1H NMR spectroscopy in conjunction with sugar analysis. After subjecting the mucins to alkaline borohydride degradation, sialylated oligosaccharide-alditols were isolated by anion-exchange chromatography and fractionated by high performance liquid chromatography. Five compounds could be obtained in a rather pure state; their structures were established as the following: A-1, NeuAc alpha(2----3)Gal beta(1----4) [Fuc alpha(1----3)]GlcNAc beta(1----3)Gal-NAc-ol; A-2, NeuAc alpha(2----3)Gal beta(1----4)GlcNAc beta(1----6)-[GlcNAc beta (1----3)]GalNAc-o1; A-3, NeuAc alpha(2----3)Gal beta-(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----3)Gal beta(1----3) GalNAc-o1; A-4, NeuAc alpha(2----3)Gal beta(1----4)[Fuc alpha(1----3)]Glc-NAc NAc beta(1----6)[GlcNAc beta(1----3)]GalNAc-o1; A-6,NeuAc alpha-(2----3) Gal beta(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----6)[Gal beta-(1----4) GlcNAc beta(1----3)]GalNAc-o1. The simultaneous presence of sialic acid in alpha(2----3)-linkage to Gal and fucose in alpha(1----3)-linkage to GlcNAc of the same N-acetyllactosamine unit could be adequately proved by high resolution 1H NMR spectroscopy. This sequence constitutes a novel structural element for mucins.
采用500兆赫的1H核磁共振光谱法并结合糖分析，对从囊性纤维化患者获取的支气管粘蛋白的唾液酸化碳水化合物单元结构进行了研究。将粘蛋白进行碱性硼氢化物降解后，通过阴离子交换色谱法分离出唾液酸化低聚糖醛糖醇，并通过高效液相色谱法进行分级分离。可以得到五种相当纯的化合物；它们的结构确定如下：A-1，NeuAcα(2→3)Galβ(1→4)[Fucα(1→3)]GlcNAcβ(1→3)Gal-NAc-ol；A-2，NeuAcα(2→3)Galβ(1→4)GlcNAcβ(1→6)-[GlcNAcβ(1→3)]GalNAc-o1；A-3，NeuAcα(2→3)Galβ-(1→4)[Fucα(1→3)]GlcNAcβ(1→3)Galβ(1→3)GalNAc-o1；A-4，NeuAcα(2→3)Galβ(1→4)[Fucα(1→3)]Glc-NAc NAcβ(1→6)[GlcNAcβ(1→3)]GalNAc-o1；A-6，NeuAcα-(2→3)Galβ(1→4)[Fucα(1→3)]GlcNAcβ(1→6)[Galβ-(1→4)GlcNAcβ(1→3)]GalNAc-o1。通过高分辨率1H核磁共振光谱法可以充分证明，在同一个N-乙酰乳糖胺单元中，唾液酸以α(2→3)连接到Gal，岩藻糖以α(1→3)连接到GlcNAc。这个序列构成了粘蛋白的一种新型结构元件。
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