Zafra R, Pérez-Ecija R A, Buffoni L, Mendes R E, Martínez-Moreno A, Martínez-Moreno F J, Galisteo M E Martínez, Pérez J
Department of Anatomy and Comparative Pathology, University of Córdoba, Faculty of Veterinary Medicine, Campus of Rabanales, Córdoba.
J Comp Pathol. 2010 Aug-Oct;143(2-3):110-9. doi: 10.1016/j.jcpa.2010.01.011. Epub 2010 Feb 24.
Worm burden, hepatic damage and local cellular and humoral immune responses were assessed in goats immunized with glutathione-S-transferase and challenged with Fasciola hepatica. Infected but unimmunized and uninfected control groups were also studied. Hepatic damage was evaluated grossly and microscopically. Local immune response was evaluated by (1) microscopical examination of hepatic lymph nodes (HLNs); (2) analysis of the distribution of CD2(+), CD4(+), CD8(+), T-cell receptor gammadelta(+) lymphocytes and immunoglobulin (Ig) G(+) plasma cells; and (3) investigation of the distribution of cells expressing interleukin (IL)-4 and interferon (IFN)-gamma in the hepatic inflammatory infiltrates and HLNs. Immunized animals did not have significant reduction in fluke number, but there was significant (P<0.05) reduction of fluke size relative to the control groups. The lesions in the two infected groups were similar and consisted of fibrous perihepatitis and white tortuous tracts, mainly involving the left hepatic lobe. Microscopical lesions were similar in both infected groups and were typical of chronic fascioliosis. These included portal fibrosis, inflammatory infiltration with plasma cells, formation of lymphoid follicles, accumulation of haemosiderin-laden macrophages and granulomatous foci. Both infected groups had a marked local immune response characterized by infiltration of CD2(+), CD4(+) and CD8(+) T lymphocytes, and IgG(+) plasma cells in hepatic lesions and in HLNs. There was no expression of IL-4 or INF-gamma by cells in the hepatic inflammatory infiltrate, but expression of INF-gamma in HLNs was much lower than that of IL-4, suggesting an immune response dominated by T helper 2 cells.
对用谷胱甘肽 - S - 转移酶免疫并经肝片吸虫攻击的山羊，评估其虫负荷、肝损伤以及局部细胞和体液免疫反应。还研究了受感染但未免疫和未受感染的对照组。通过大体和显微镜检查评估肝损伤。通过以下方式评估局部免疫反应：（1）对肝淋巴结（HLN）进行显微镜检查；（2）分析CD2(+)、CD4(+)、CD8(+)、T细胞受体γδ(+)淋巴细胞和免疫球蛋白（Ig）G(+)浆细胞的分布；（3）研究在肝脏炎性浸润和HLN中表达白细胞介素（IL）-4和干扰素（IFN）-γ的细胞分布。免疫动物的吸虫数量没有显著减少，但相对于对照组，吸虫大小显著（P<0.05）减小。两个感染组的病变相似，包括纤维性肝周炎和白色迂曲条索，主要累及左肝叶。两个感染组的显微镜下病变相似，是慢性肝片吸虫病的典型表现。这些病变包括门脉纤维化、浆细胞炎性浸润、淋巴滤泡形成、含铁血黄素巨噬细胞积聚和肉芽肿灶。两个感染组均有明显的局部免疫反应，其特征是肝病变和HLN中有CD2(+)、CD4(+)和CD8(+) T淋巴细胞以及IgG(+)浆细胞浸润。肝脏炎性浸润中的细胞未表达IL - 4或INF - γ，但HLN中INF - γ的表达远低于IL - 4，表明免疫反应以辅助性T细胞2为主导。
