Buffoni L, Zafra R, Pérez-Ecija A, Martínez-Moreno F J, Martínez-Galisteo E, Moreno T, Pérez J, Martínez-Moreno A
Animal Health Department (Parasitology), Faculty of Veterinary Medicine, University of Córdoba, Campus de Rabanales, Ctra. Madrid-Cádiz, Córdoba, Spain.
Parasitol Int. 2010 Jun;59(2):147-53. doi: 10.1016/j.parint.2009.12.005. Epub 2009 Dec 24.
Glutathione S-transferase (FhGST) purified from Fasciola hepatica adult worms was used to immunise goats against F. hepatica in an experimental infection; the level of protection, in terms of fluke burden, faecal egg counts and hepatic damage was determined, as well as the humoral and cellular immune response elicited. Animals were allocated into three groups of six animals each: group 1 (immunised with FhGST and infected), group 2 (unimmunised and infected), and group 3 (unimmunised and uninfected). There was no significant reduction of fluke burden (9.3%) or faecal egg counts; hepatic damage was also similar in both infected groups. However, immunisation with FhGST induced the development of a well-defined immune response, characterized by the production of specific-FhGST antibodies as well as the appearance of circulating IL-4.
从肝片吸虫成虫中纯化的谷胱甘肽S-转移酶（FhGST）被用于在实验性感染中免疫山羊以抵抗肝片吸虫；测定了在吸虫负荷、粪便虫卵计数和肝损伤方面的保护水平，以及引发的体液和细胞免疫反应。将动物分为三组，每组六只动物：第1组（用FhGST免疫并感染）、第2组（未免疫并感染）和第3组（未免疫且未感染）。吸虫负荷（9.3%）或粪便虫卵计数没有显著降低；两个感染组的肝损伤也相似。然而，用FhGST免疫诱导了明确的免疫反应的发展，其特征是产生特异性FhGST抗体以及循环IL-4的出现。
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