Department of Anatomy and Comparative Pathology, School of Veterinary Medicine, University of Cordoba, Animal Health Building, Campus Rabanales, Cordoba, Spain.
Vaccine. 2010 Apr 1;28(16):2832-40. doi: 10.1016/j.vaccine.2010.01.055. Epub 2010 Feb 12.
Protection against Fasciola hepatica in goats immunized with Peroxiredoxin (Prx) was assessed. The experimental trial consisted of three groups of seven animals; group 1 were unimmunized and uninfected, group 2 were immunized with adjuvant only and group 3 were immunized with recombinant Prx in adjuvant (immunized and infected). Immunization with Prx in Quil A adjuvant, group 3, induced a reduction in fluke burden of 33.04% when compared to adjuvant control, group 2, although this difference was not significant. The hepatic gross and microscopical morphometric study revealed lower damage in the Prx-immunized compared to group 2 (p<0.05). Furthermore, immunohistochemical studies revealed that the Prx-immunized group exhibited reduced infiltration of CD4(+), CD8(+), IFN-gamma(+) and TCR(+) (p<0.05); and CD2(+) and IL-4(+) (p<0.001) in hepatic lesions. Levels of anti-Prx serum IgG in group 3 showed a significant increase at the 4th week after challenge infection compared with group 2 (p<0.0001). This is the first report of ruminant immunization with recombinant Prx of F. hepatica. The study shows that this vaccine significantly reduces hepatic damage and encourages further studies to improve the vaccine efficacy.
评估了用过氧化物还原酶（Prx）免疫山羊对肝片吸虫的保护作用。该实验包括三组 7 只动物；第 1 组未免疫且未感染，第 2 组仅用佐剂免疫，第 3 组用佐剂中的重组 Prx 免疫（免疫和感染）。用 Quil A 佐剂免疫 Prx 组，与佐剂对照组第 2 组相比，降低了 33.04%的吸虫负担，尽管这一差异无统计学意义。肝大体和显微镜形态计量研究表明，与第 2 组相比，Prx 免疫组的损伤较小（p<0.05）。此外，免疫组织化学研究表明，Prx 免疫组在肝损伤中显示出 CD4(+)、CD8(+)、IFN-γ(+)和 TCR(+)浸润减少（p<0.05）；CD2(+)和 IL-4(+)浸润减少（p<0.001）。与第 2 组相比，第 3 组在攻毒感染后第 4 周时的抗 Prx 血清 IgG 水平显著升高（p<0.0001）。这是首次报道用重组肝片吸虫 Prx 对反刍动物进行免疫。研究表明，这种疫苗能显著减轻肝损伤，并鼓励进一步研究以提高疫苗的功效。
