Heart Failure Research Center, Academic Medical Center, Meibergdreef 15, Amsterdam, The Netherlands.
Circ Res. 2010 Apr 2;106(6):1035-9. doi: 10.1161/CIRCRESAHA.110.218297. Epub 2010 Feb 25.
Aberrant expression profiles of circulating microRNAs (miRNAs) have been described in various diseases and provide high sensitivity and specificity. We explored circulating miRNAs as potential biomarkers in patients with heart failure (HF).
The goal of this study was to determine whether miRNAs allow to distinguish clinical HF not only from healthy controls but also from non-HF forms of dyspnea.
A miRNA array was performed on plasma of 12 healthy controls and 12 HF patients. From this array, we selected 16 miRNAs for a second clinical study in 39 healthy controls and in 50 cases with reports of dyspnea, of whom 30 were diagnosed with HF and 20 were diagnosed with dyspnea attributable to non-HF-related causes. This revealed that miR423-5p was specifically enriched in blood of HF cases and receiver-operator-characteristics (ROC) curve analysis showed miR423-5p to be a diagnostic predictor of HF, with an area under the curve of 0.91 (P<0.001). Five other miRNAs were elevated in HF cases but also slightly increased in non-HF dyspnea cases.
We identify 6 miRNAs that are elevated in patients with HF, among which miR423-5p is most strongly related to the clinical diagnosis of HF. These 6 circulating miRNAs provide attractive candidates as putative biomarkers for HF.
循环 microRNAs（miRNAs）的异常表达谱已在各种疾病中得到描述，并具有较高的灵敏度和特异性。我们探讨了循环 miRNAs 是否可作为心力衰竭（HF）患者的潜在生物标志物。
本研究旨在确定 miRNAs 是否不仅可区分有临床症状的 HF 患者与健康对照者，而且可区分非 HF 呼吸困难患者。
对 12 名健康对照者和 12 名 HF 患者的血浆进行 miRNA 芯片分析。在此芯片的基础上，我们选择了 16 个 miRNA 进行第二次临床研究，纳入了 39 名健康对照者和 50 名有呼吸困难报告的患者，其中 30 名患者被诊断为 HF，20 名患者被诊断为非 HF 相关原因引起的呼吸困难。结果表明，miR423-5p 特异性富集在 HF 患者的血液中，受试者工作特征（ROC）曲线分析显示 miR423-5p 是 HF 的诊断预测因子，曲线下面积为 0.91（P<0.001）。另外 5 个 miRNA 在 HF 病例中升高，但在非 HF 呼吸困难病例中也略有升高。
我们鉴定出 6 个在 HF 患者中升高的 miRNA，其中 miR423-5p 与 HF 的临床诊断最密切相关。这 6 个循环 miRNA 作为潜在的 HF 生物标志物具有吸引力。
