Kakigi Akinobu, Uchida Aya, Nishimura Masahiko, Takeda Taizo, Takeda Setsuko, Nakatani Hiroaki
Department of Otolaryngology, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
ORL J Otorhinolaryngol Relat Spec. 2010;71 Suppl 1:67-70. doi: 10.1159/000265121. Epub 2010 Feb 24.
To locate fibroblast growth factor receptor (FGFRs) 1-4 in human chronic tympanic membrane (TM) perforation.
A sample of human chronic TM perforation was harvested during myringoplasty. The sample was immediately fixed in 4% paraformaldehyde and embedded in OCT compound. Immunohistochemistry was performed with FGFR 1-4 polyclonal antibodies.
FGFRs 1-4 were strongly and weakly expressed in the epidermal and mucosal layer of the TM perforation, respectively.
As it is impossible to perform quantitative analysis based on the fluorescence intensity of each immunoreactivity, the presence of FGFRs 1-4 in the human chronic TM perforation is shown. The expressions of FGFRs 1-4 indicated that the clinical use of bFGF agent is useful for myringoplasty.
在人类慢性鼓膜穿孔中定位成纤维细胞生长因子受体（FGFRs）1 - 4。
在鼓膜成形术期间获取人类慢性鼓膜穿孔样本。样本立即固定于4%多聚甲醛中并包埋于OCT复合物中。用FGFR 1 - 4多克隆抗体进行免疫组织化学。
FGFRs 1 - 4分别在鼓膜穿孔的表皮层和黏膜层中强表达和弱表达。
由于无法基于每种免疫反应性的荧光强度进行定量分析，所以显示了FGFRs 1 - 4在人类慢性鼓膜穿孔中的存在。FGFRs 1 - 4的表达表明碱性成纤维细胞生长因子（bFGF）制剂在鼓膜成形术中的临床应用是有效的。
