血管内皮生长因子在急性鼓膜穿孔愈合中的重要性。
The importance of vascular endothelial growth factor in the healing of acute tympanic membrane perforation.
机构信息
Department of Otorhinolaryngology, Pusan National University School of Medicine, Busan, South Korea.
出版信息
Am J Otolaryngol. 2010 Sep-Oct;31(5):309-14. doi: 10.1016/j.amjoto.2009.03.005. Epub 2009 Jun 4.
PURPOSE
The purpose of this study was to determine the more important growth factor expression between basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in the healing of acute tympanic membrane (TM) perforation.
MATERIALS AND METHODS
Bilateral perforations of the TM were created in 12 rats. The TM perforations in the right ears were treated with dexamethasone, and left ears were designated as the control group. The TM was examined for the growth factor expression immunohistochemically in the epithelial and fibrous layers according to the rate of TM perforation healing.
RESULTS
The mean spontaneous healing time of the TM perforations was 11.0 +/- 2.0 days. However, dexamethasone-treated group showed no evidence of closure. The bFGF and VEGF expression were significantly correlated with the rate of healing of acute TM perforations. The VEGF expression was decreased both in the epithelial and fibrous layers, but bFGF expression was decreased only in the epithelial layer in the dexamethasone-treated group. The VEGF was expressed to a lesser degree than bFGF in the dexamethasone-treated group.
CONCLUSION
Vascular endothelial growth factor is the more specific and important growth factor than bFGF in the healing of acute TM perforation.
目的
本研究旨在确定碱性成纤维细胞生长因子(bFGF)和血管内皮生长因子(VEGF)在急性鼓膜穿孔愈合中的更重要的生长因子表达。
材料与方法
在 12 只大鼠中造成双侧鼓膜穿孔。右耳鼓膜穿孔用地塞米松治疗，左耳为对照组。根据鼓膜穿孔愈合率，用免疫组织化学方法检测上皮和纤维层中的生长因子表达。
结果
鼓膜穿孔的平均自发愈合时间为 11.0±2.0 天。然而，地塞米松治疗组没有愈合的迹象。bFGF 和 VEGF 的表达与急性鼓膜穿孔的愈合率显著相关。VEGF 在上皮和纤维层中的表达均降低，但地塞米松治疗组中 bFGF 在上皮层中的表达降低。地塞米松治疗组中 VEGF 的表达程度低于 bFGF。
结论
在急性鼓膜穿孔愈合中，VEGF 是比 bFGF 更特异和重要的生长因子。
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