人神经母细胞瘤中酪氨酸激酶受体与血管内皮生长因子基因表达的相关性研究。

The studies on the correlation for gene expression of tyrosine-kinase receptors and vascular endothelial growth factor in human neuroblastomas.

作者信息

Zhang Jihong, Zheng Yingchun, Wang Yunxiu, Tong Haixia

机构信息

Department of Hematology/Oncology Laboratory, Sheng Jing Hospital Affiliated to China Medical University, Shenyang, China.

出版信息

J Pediatr Hematol Oncol. 2010 Apr;32(3):180-4. doi: 10.1097/MPH.0b013e3181c46bab.

DOI:10.1097/MPH.0b013e3181c46bab

PMID:20186105

Abstract

OBJECTIVE

To study the correlation and clinical significance of expression of tyrosine-kinase receptors (TrkA and TrkB) and vascular endothelial growth factor (VEGF) in human neuroblastomas.

METHODS

Expression of TrkA, TrkB, and VEGF mRNA was semi-quantitatively detected by reverse transcription-polymerase chain reaction (RT-PCR) in 51 cases of neuroblastomas.

RESULTS

The expression of TrkA was significantly higher in lower-stage group compared with higher-stage group (P<0.05), whereas the expression of VEGF was significantly higher in the higher-stage group compared with the lower-stage group (P<0.05). The expression of TrkA was correlated negatively with the expression of VEGF (P<0.01), and has remarkable dependability with 2-year cumulative survival rate (P<0.01). The expression of TrkA in the lower age group was significantly higher than in the higher age group of NB cases (P<0.01). TrkA has a good prognostic impact on neuroblastoma patients (P<0.01). The expression of TrkB was significantly higher in the higher-stage group compared with the lower-stage group (P<0.05) and was positively correlated with VEGF expression (r=0.342, P<0.05); their expression also has remarkable dependability with the 2-year cumulative survival rate (P<0.01). The expression of TrkB was significantly lower in the higher age group compared with the lower age group (P<0.05). The 2-year cumulative-survival rate in the lower age group had a great significance compared with the higher age group (P<0.001). TrkB has a bad prognostic impact on neuroblastoma patients (P<0.01).

CONCLUSIONS

TrkA was highly expressed in good prognostic neuroblastomas; however, TrkB and VEGF were highly expressed in poor prognostic neuroblastomas. The expression of TrkA was negatively correlated with the expression of VEGF, whereas the expression of TrkB was positively correlated with the expression of VEGF. These 3 genes have an important clinical significance relating to the tumor stage and the outcome for patients with neuroblastomas.

摘要

目的

研究酪氨酸激酶受体（TrkA和TrkB）及血管内皮生长因子（VEGF）在人神经母细胞瘤中的表达相关性及临床意义。

方法

采用逆转录聚合酶链反应（RT-PCR）半定量检测51例神经母细胞瘤中TrkA、TrkB和VEGF mRNA的表达。

结果

TrkA在低分期组中的表达显著高于高分期组（P<0.05），而VEGF在高分期组中的表达显著高于低分期组（P<0.05）。TrkA的表达与VEGF的表达呈负相关（P<0.01），且与2年累积生存率具有显著相关性（P<0.01）。TrkA在低年龄组神经母细胞瘤病例中的表达显著高于高年龄组（P<0.01）。TrkA对神经母细胞瘤患者具有良好的预后影响（P<0.01）。TrkB在高分期组中的表达显著高于低分期组（P<0.05），且与VEGF表达呈正相关（r=0.342，P<0.05）；它们的表达也与2年累积生存率具有显著相关性（P<0.01）。TrkB在高年龄组中的表达显著低于低年龄组（P<0.05）。低年龄组的2年累积生存率与高年龄组相比具有显著差异（P<0.001）。TrkB对神经母细胞瘤患者具有不良的预后影响（P<0.01）。