Department of Public Health and Clinical Medicine, Umeå University Hospital, Umeå, Sweden.
Obesity (Silver Spring). 2010 May;18(5):879-83. doi: 10.1038/oby.2010.22.
Obesity can be considered as a low-grade inflammatory condition, strongly linked to adverse metabolic outcomes. Obesity-associated adipose tissue inflammation is characterized by infiltration of macrophages and increased cytokine and chemokine production. The distribution of adipose tissue impacts the outcomes of obesity, with the accumulation of fat in visceral adipose tissue (VAT) and deep subcutaneous adipose tissue (SAT), but not superficial SAT, being linked to insulin resistance. We hypothesized that the inflammatory gene expression in deep SAT and VAT is higher than in superficial SAT. A total of 17 apparently healthy women (BMI: 29.3 +/- 5.5 kg/m2) were included in the study. Body fat (dual-energy X-ray absorptiometry) and distribution (computed tomography) were measured, and insulin sensitivity, blood lipids, and blood pressure were determined. Inflammation-related differences in gene expression(real-time PCR) from VAT, superficial and deep SAT biopsies were analyzed using univariate and multivariate data analyses. Using multivariate discrimination analysis, VAT appeared as a distinct depot in adipose tissue inflammation,while the SAT depots had a similar pattern, with respect to gene expression. A significantly elevated (P < 0.01)expression of the CC chemokine receptor 2 (CCR2) and macrophage migration inhibitory factor (MIF) in VAT contributed strongly to the discrimination. In conclusion, the human adipose tissue depots have unique inflammatory patterns, with CCR2 and MIF distinguishing between VAT and the SAT depots.
肥胖可以被视为一种低度炎症状态，与不良代谢后果密切相关。与肥胖相关的脂肪组织炎症的特征是巨噬细胞浸润和细胞因子及趋化因子产生增加。脂肪组织的分布影响肥胖的结果，内脏脂肪组织（VAT）和深部皮下脂肪组织（SAT）中脂肪的积累与胰岛素抵抗有关，但浅层 SAT 中脂肪的积累与胰岛素抵抗无关。我们假设深部 SAT 和 VAT 中的炎症基因表达高于浅层 SAT。共有 17 名明显健康的女性（BMI：29.3 +/- 5.5 kg/m2）纳入研究。测量体脂肪（双能 X 射线吸收法）和分布（计算机断层扫描），并测定胰岛素敏感性、血脂和血压。使用单变量和多变量数据分析，分析来自 VAT、浅层和深部 SAT 活检的与炎症相关的基因表达差异（实时 PCR）。使用多元判别分析，VAT 作为脂肪组织炎症的独特储存库出现，而 SAT 储存库在基因表达方面具有相似的模式。CC 趋化因子受体 2（CCR2）和巨噬细胞移动抑制因子（MIF）的表达显著升高（P < 0.01），这对判别有很大贡献。总之，人类脂肪组织储存库具有独特的炎症模式，CCR2 和 MIF 可区分 VAT 和 SAT 储存库。
