Paerregaard A, Espersen F, Skurnik M
Institute of Medical Microbiology, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1991 Mar;99(3):226-32. doi: 10.1111/j.1699-0463.1991.tb05143.x.
The Yersinia virulence plasmid confers on strains of Yersinia pseudotuberculosis and Y. enterocolitica an adhesive potential superior to the one encoded by the chromosome alone. We have evaluated the role of the plasmid-encoded outer membrane protein YadA (formerly called Yopl) in adhesion. Insertional inactivation of the yadA gene (formerly called yopA), which encodes YadA, led to a reduction in the capacity of plasmid-carrying strains of Y. pseudotuberculosis 0:III and Y. enterocolitica 0:9 to adhere to intestinal tissue, brush border membranes and polystyrene surfaces. The adhesive characteristics of the mutants were comparable to those of their plasmid-cured counterparts. When the yadA gene from Y. pseudotuberculosis serotype 0:III or Y. enterocolitica serotype 0:3 or 0:8 was cloned into an Escherichia coli strain, increased ability to adhere to intestinal tissue, brush border membrane vesicles and polystyrene was transferred concomitantly. The introduction of the yadA gene from Y. pestis, which is unable to express YadA due to a one base pair deletion, did not change the adhesive characteristics of E. coli. Expression of YadA in the outer membrane may, therefore, make an important contribution to intestinal adherence of the two enteropathogenic members of the Yersinia species, Y. pseudotuberculosis and Y. enterocolitica.
耶尔森氏菌毒力质粒赋予假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌菌株的黏附能力优于仅由染色体编码的黏附能力。我们评估了质粒编码的外膜蛋白YadA（以前称为Yopl）在黏附中的作用。编码YadA的yadA基因（以前称为yopA）的插入失活导致携带质粒的假结核耶尔森氏菌0:III菌株和小肠结肠炎耶尔森氏菌0:9菌株黏附肠道组织、刷状缘膜和聚苯乙烯表面的能力降低。突变体的黏附特性与其质粒消除后的对应物相当。当将来自假结核耶尔森氏菌血清型0:III或小肠结肠炎耶尔森氏菌血清型0:3或0:8的yadA基因克隆到大肠杆菌菌株中时，同时转移了其黏附肠道组织、刷状缘膜囊泡和聚苯乙烯的能力增强。由于一个碱基对缺失而无法表达YadA的鼠疫耶尔森氏菌的yadA基因的引入并没有改变大肠杆菌的黏附特性。因此，YadA在外膜中的表达可能对耶尔森氏菌属的两种肠道致病性成员，即假结核耶尔森氏菌和小肠结肠炎耶尔森氏菌的肠道黏附起重要作用。
