Skurnik M, el Tahir Y, Saarinen M, Jalkanen S, Toivanen P
Turku Centre for Biotechnology, Finland.
Infect Immun. 1994 Apr;62(4):1252-61. doi: 10.1128/iai.62.4.1252-1261.1994.
The binding of live Yersinia enterocolitica to frozen sections of human intestine was investigated qualitatively by monitoring the binding of bacteria by using Gram or immunoperoxidase staining as well as quantitatively by a new enzyme immunoassay-on-slide method. We have demonstrated that the binding of various Y. enterocolitica serotypes and Escherichia coli clones to frozen sections of human intestine is mediated by the Yersinia adhesin, YadA. The YadA-mediated binding occurs mainly at the submucosal layer of the intestinal wall and only to a limited extent at the mucosal layer; there binding is mostly to the mucin threads. In addition, partially purified YadA binds to frozen sections with a pattern similar to that of intact bacteria. Collagen, laminin, or partially purified YadA only partially inhibited the YadA-mediated binding of bacteria, presumably because YadA is multifunctional. A combination of collagen and laminin inhibited the binding more efficiently. Therefore, YadA may be involved in the interactions with the extracellular matrix molecules after the invasion of the intestinal tissue.
通过革兰氏染色或免疫过氧化物酶染色监测细菌结合情况对活的小肠结肠炎耶尔森菌与人肠道冰冻切片的结合进行定性研究，并采用一种新的玻片酶免疫测定法进行定量研究。我们已经证明，各种小肠结肠炎耶尔森菌血清型和大肠杆菌克隆与人肠道冰冻切片的结合是由耶尔森菌粘附素YadA介导的。YadA介导的结合主要发生在肠壁的粘膜下层，仅在粘膜层有有限程度的结合；在那里结合主要是与粘蛋白丝结合。此外，部分纯化的YadA以与完整细菌相似的模式结合到冰冻切片上。胶原蛋白、层粘连蛋白或部分纯化的YadA仅部分抑制YadA介导的细菌结合，推测是因为YadA具有多种功能。胶原蛋白和层粘连蛋白的组合更有效地抑制了结合。因此，YadA可能在肠道组织入侵后参与与细胞外基质分子的相互作用。
