Wikström M, Jonsson G, Svennerholm A M
Department of Medical Microbiology and Immunology, University of Göteborg, Sweden.
APMIS. 1991 Mar;99(3):249-56.
Monoclonal antibodies (MAbs) against a protease of Vibrio cholerae, the soluble haemagglutinin (sHA), have been prepared and characterized with regard to their ability to inhibit different biological properties of sHA and to protect against experimental V. cholerae infection. Four fusion experiments yielded two specific immunoglobulin G1 MAbs that reacted with sHA produced by different V. cholerae O1 and non-O1 strains but that differed in their antigen-binding capacity. Both MAbs were capable of inhibiting the haemagglutinating and protease activities of sHA as well as its ability to nick the enterotoxin A subunit, but neither of them had any effect on the mucinase activity of sHA. One of the MAbs significantly inhibited experimental cholera infection induced by sHA-producing V. cholerae O1 in rabbit small intestine.
已制备出针对霍乱弧菌一种蛋白酶——可溶性血凝素（sHA）的单克隆抗体（MAb），并对其抑制sHA不同生物学特性以及预防实验性霍乱弧菌感染的能力进行了表征。四次融合实验产生了两种特异性免疫球蛋白G1单克隆抗体，它们与不同霍乱弧菌O1和非O1菌株产生的sHA发生反应，但抗原结合能力不同。两种单克隆抗体均能够抑制sHA的血凝和蛋白酶活性及其切割肠毒素A亚基的能力，但它们对sHA的粘蛋白酶活性均无任何影响。其中一种单克隆抗体显著抑制了产sHA的霍乱弧菌O1在兔小肠中诱导的实验性霍乱感染。
