细胞相关的N-乙酰-D-葡萄糖胺特异性血凝素在霍乱弧菌O1体外黏附兔肠道上皮细胞中的作用。
Role of cell-associated N-acetyl-D-glucosamine specific haemagglutinin in the adhesion of Vibrio cholerae O1 to rabbit intestinal epithelial cells in vitro.
作者信息
Sasmal D, Guhathakurta B, Bhattacharya S K, Pal C R, Datta A
机构信息
National Institute of Cholera and Enteric Diseases, Calcutta, India.
出版信息
FEMS Immunol Med Microbiol. 1996 Feb;13(2):101-5. doi: 10.1016/0928-8244(95)00090-9.
Previously a N-acetyl-D-glucosamine specific cell-associated haemagglutinin (HA) had been purified from a Vibrio cholerae O1 strain. This study documents the role of this purified HA as an adhesin of V. cholerae O1. A significant inhibition in the adhesion of V. cholerae O1 bacterial cells to isolated rabbit intestinal brush borders (RIBB) was observed when the latter were pretreated with purified HA in ELISA. Antibody raised against purified HA and Fab (IgG) fragment of this serum inhibited adhesion of the bacteria to isolated rabbit intestinal epithelial cells (RIEC). V. cholerae O1 (both Ogawa and Inaba serovars) showed less adherence to isolated RIEC of animals immunised with the purified HA. Patients convalescing from V. cholerae O1 infection showed high ELISA titres against the purified HA indicating that it is expressed in the host during the disease process.
先前已从一株霍乱弧菌O1中纯化出一种N - 乙酰 - D - 葡糖胺特异性细胞相关血凝素（HA）。本研究记录了这种纯化的HA作为霍乱弧菌O1黏附素的作用。在酶联免疫吸附测定（ELISA）中，当用纯化的HA预处理分离的兔肠刷状缘（RIBB）时，观察到霍乱弧菌O1细菌细胞对其黏附的显著抑制。针对纯化的HA产生的抗体以及该血清的Fab（IgG）片段抑制了细菌对分离的兔肠上皮细胞（RIEC）的黏附。霍乱弧菌O1（小川型和稻叶型血清型）对用纯化的HA免疫的动物的分离RIEC的黏附较少。从霍乱弧菌O1感染中康复的患者对纯化的HA显示出高ELISA滴度，表明它在疾病过程中在宿主体内表达。
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