College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Funct Integr Genomics. 2010 May;10(2):227-39. doi: 10.1007/s10142-010-0161-8. Epub 2010 Feb 26.
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. To isolate defense-related genes against the pathogen, a suppression subtractive hybridization library was constructed for an incompatible interaction. From the library, 652 sequences were determined to be unigenes, of which 31 were determined as genes involved in signal transduction and 77 were predicted to encode defense-related proteins. Expression patterns of 12 selected signal transduction and defense-related genes were determined using quantitative real-time polymerase chain reaction. Signal transduction genes started increasing their expression at 12 h post inoculation (hpi), and expressions of the most of the transport and resistance-related genes were induced at 18 hpi. The gene expression results indicate specific molecular and cellular activities during the incompatible interaction between wheat and the stripe rust pathogen. In general, the expression increase of wheat signal transduction genes soon after inoculation with the pathogen inducing various defense-related genes, including reactive oxygen species, ATP-binding cassette (ABC) transporters, pathogenesis-related proteins, and genes involved in the phenylpropanoid pathway. The activities of these defense genes work in a sequential and concerted manner to result in a hypersensitive response.
小麦条锈病由条形柄锈菌小麦专化型引起，是全球小麦最重要的病害之一。为了分离针对病原体的防御相关基因，我们构建了一个不亲和互作的抑制差减杂交文库。从文库中鉴定了 652 个序列为单基因，其中 31 个被确定为参与信号转导的基因，77 个被预测为编码防御相关蛋白的基因。使用定量实时聚合酶链反应（PCR）测定了 12 个选定的信号转导和防御相关基因的表达模式。信号转导基因在接种后 12 小时（hpi）开始增加表达，大多数运输和抗性相关基因在 18 hpi 时被诱导。基因表达结果表明在小麦与条锈病菌不亲和互作过程中存在特定的分子和细胞活动。一般来说，在接种病原体后，小麦信号转导基因的表达很快增加，诱导各种防御相关基因的表达，包括活性氧、ATP 结合盒（ABC）转运蛋白、病程相关蛋白和苯丙烷途径相关基因。这些防御基因的活性以顺序和协同的方式发挥作用，导致过敏反应。
