利用电化学 DNA 生物传感器检测芴的硝基衍生物对 DNA 的损伤。
Voltammetric detection of damage to DNA caused by nitro derivatives of fluorene using an electrochemical DNA biosensor.
机构信息
Charles University in Prague, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Hlavova 2030/8, 12843, Prague 2, Czech Republic.
Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Radlinského 9, 81237, Bratislava, Slovakia.
出版信息
Anal Bioanal Chem. 2010 May;397(1):233-241. doi: 10.1007/s00216-010-3517-y. Epub 2010 Feb 26.
An electrochemical DNA biosensor based on the screen printed carbon paste electrode (SPCPE) with an immobilized layer of calf thymus double-stranded DNA has been used for in vitro investigation of the interaction between genotoxic nitro derivatives of fluorene (namely 2-nitrofluorene and 2,7-dinitrofluorene) and DNA. Two types of DNA damage have been detected at the DNA/SPCPE biosensor: first, that caused by direct association of the nitrofluorenes, for which an intercalation association has been found using the known DNA intercalators Cu(phen)(2) and Co(phen)(3) as competing agents, and, second, that caused by short-lived radicals generated by electrochemical reduction of the nitro group (observable under specific conditions only).
基于涂覆碳糊电极（SPCPE）的电化学 DNA 生物传感器，其中固定了一层小牛胸腺双链 DNA，已用于体外研究芴的遗传毒性硝基衍生物（即 2-硝基芴和 2,7-二硝基芴）与 DNA 之间的相互作用。在 DNA/SPCPE 生物传感器上检测到两种类型的 DNA 损伤：首先，由硝基芴的直接缔合引起的损伤，对于这种损伤，已经发现了一种嵌入缔合，使用已知的 DNA 嵌入剂[Cu(phen)(2)]（2+）和[Co(phen)(3)]（3+）作为竞争剂；其次，由硝基电化学还原产生的短寿命自由基引起的损伤（仅在特定条件下观察到）。
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