用于分析废水样本的电化学DNA生物传感器。

Electrochemical DNA biosensor for analysis of wastewater samples.

作者信息

Lucarelli F, Kicela A, Palchetti I, Marrazza G, Mascini M

机构信息

Dipartimento di Chimica/Polo Scientifico, Università degli studi di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.

出版信息

Bioelectrochemistry. 2002 Nov;58(1):113-8. doi: 10.1016/s1567-5394(02)00133-0.

DOI:10.1016/s1567-5394(02)00133-0

PMID:12401577

Abstract

The application of a disposable electrochemical DNA biosensor to wastewater samples is reported. The DNA biosensor is assembled by immobilising double-stranded calf thymus DNA on the surface of a disposable, carbon screen-printed electrode (SPE). The oxidation signal of the guanine base, obtained by a square wave voltammetric scan, is used as analytical signal. The presence of compounds with affinity for DNA is measured by their effect on the guanine oxidation. The comparison of the results with a toxicity test based on bioluminescent bacteria has confirmed the applicability of the method to real samples.

摘要

报道了一种一次性电化学DNA生物传感器在废水样品中的应用。该DNA生物传感器是通过将双链小牛胸腺DNA固定在一次性碳丝网印刷电极（SPE）表面组装而成。通过方波伏安扫描获得的鸟嘌呤碱基的氧化信号用作分析信号。对DNA具有亲和力的化合物的存在通过它们对鸟嘌呤氧化的影响来测定。将结果与基于生物发光细菌的毒性测试进行比较，证实了该方法对实际样品的适用性。