Department of Trauma Surgery, Hannover Medical School, Hannover D-30625, Germany.
J Biomed Mater Res A. 2010 Aug;94(2):442-9. doi: 10.1002/jbm.a.32696.
Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matrix-producing osteoblasts. Collagen type I is of special interest as it is a very important and abundant natural matrix component. The purpose of this study was to investigate whether rat bone marrow stromal cells (rBMSCs) are able to adhere to, to survive, to proliferate and to migrate in collagen type I hydrogels and whether they can adopt an osteoblastic fate. rBMSCs were obtained from rat femora and plated on collagen type I hydrogels. Before harvest by day 7, 14, and 21, hydrogels were fluorescently labeled, cryo-cut and analyzed by fluorescent-based and laser scanning confocal microscopy to determine cell proliferation, migration, and viability. Osteogenic differentiation was determined by alkaline phosphatase activity. Collagen type I hydrogels allowed the attachment of rBMSCs to the hydrogel, their proliferation, and migration towards the inner part of the gel. rBMSCs started to differentiate into osteoblasts as determined by an increase in alkaline phosphatase activity after two weeks in culture. This study therefore suggests that collagen type I hydrogels could be useful for musculoskeletal regenerative therapies.
水凝胶在再生医学中有广泛的应用潜力，包括药物和生长因子的递送、作为单一支架用于骨修复，或者作为另一种生物材料的孔的填充物，其中宿主间充质祖细胞可以迁移并分化为产生基质的成骨细胞。I 型胶原特别引人注目，因为它是一种非常重要且丰富的天然基质成分。本研究旨在探讨大鼠骨髓基质细胞（rBMSCs）是否能够黏附、存活、增殖和迁移到 I 型胶原水凝胶中，以及它们是否能够采用成骨细胞命运。rBMSCs 从大鼠股骨中分离出来并种植在 I 型胶原水凝胶上。在第 7、14 和 21 天收获之前，用水凝胶进行荧光标记，冷冻切片，并通过荧光和激光共聚焦显微镜分析，以确定细胞增殖、迁移和活力。碱性磷酸酶活性用于确定成骨分化。I 型胶原水凝胶允许 rBMSCs 附着在水凝胶上，并向凝胶内部迁移和增殖。rBMSCs 在培养两周后碱性磷酸酶活性增加，开始向成骨细胞分化。因此，本研究表明 I 型胶原水凝胶可能对肌肉骨骼再生治疗有用。
