Ahmed Tamer A E, Dare Emma V, Hincke Max
Department of Cellular and Molecular Medicine, University of Ottawa, Ontario, Canada.
Tissue Eng Part B Rev. 2008 Jun;14(2):199-215. doi: 10.1089/ten.teb.2007.0435.
Tissue engineering combines cell and molecular biology with materials and mechanical engineering to replace damaged or diseased organs and tissues. Fibrin is a critical blood component responsible for hemostasis, which has been used extensively as a biopolymer scaffold in tissue engineering. In this review we summarize the latest developments in organ and tissue regeneration using fibrin as the scaffold material. Commercially available fibrinogen and thrombin are combined to form a fibrin hydrogel. The incorporation of bioactive peptides and growth factors via a heparin-binding delivery system improves the functionality of fibrin as a scaffold. New technologies such as inkjet printing and magnetically influenced self-assembly can alter the geometry of the fibrin structure into appropriate and predictable forms. Fibrin can be prepared from autologous plasma, and is available as glue or as engineered microbeads. Fibrin alone or in combination with other materials has been used as a biological scaffold for stem or primary cells to regenerate adipose tissue, bone, cardiac tissue, cartilage, liver, nervous tissue, ocular tissue, skin, tendons, and ligaments. Thus, fibrin is a versatile biopolymer, which shows a great potential in tissue regeneration and wound healing.
组织工程学将细胞与分子生物学和材料及机械工程相结合，以替代受损或患病的器官和组织。纤维蛋白是负责止血的关键血液成分，在组织工程中已被广泛用作生物聚合物支架。在本综述中，我们总结了使用纤维蛋白作为支架材料在器官和组织再生方面的最新进展。市售的纤维蛋白原和凝血酶结合形成纤维蛋白水凝胶。通过肝素结合递送系统掺入生物活性肽和生长因子可改善纤维蛋白作为支架的功能。诸如喷墨打印和磁影响自组装等新技术可将纤维蛋白结构的几何形状改变为合适且可预测的形式。纤维蛋白可从自体血浆中制备，有胶水或工程微珠等形式。单独的纤维蛋白或与其他材料结合已被用作干细胞或原代细胞的生物支架，用于再生脂肪组织、骨骼、心脏组织、软骨、肝脏、神经组织、眼部组织、皮肤、肌腱和韧带。因此，纤维蛋白是一种多功能生物聚合物，在组织再生和伤口愈合方面具有巨大潜力。
