Division of Medical Devices, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
J Biomed Mater Res A. 2010 Aug;94(2):524-32. doi: 10.1002/jbm.a.32714.
A surface of biomaterials is known to affect the behavior of cells after their adhesion on the surface, indicating that surface characteristics of biomaterials play an important role in cell adhesion, proliferation, and differentiation. To assess the effects of functional groups on biomaterial surface, normal human osteoblasts (NHOsts) were cultured on surfaces coated with self-assembled monolayers (SAMs) containing various functional groups, and the adhesion, proliferation, differentiation, and gap junctional intercellular communication (GJIC) of the NHOsts were investigated. In the case of SAM with terminal methyl groups (hydrophobic surface), NHOst adhesion and proliferation was less prevalent. In contrast, NHOsts were adhered well on SAMs with hydroxyl, carboxyl, amino, phosphate, and sulfate group, which are relatively hydrophilic, their proliferation and differentiation level were dependent on the type of functional groups. Especially, when they were cultured on either SAMs with phosphate or sulfate group, both their alkaline phosphate activity and the calcium deposition by them were enhanced more than those cultured on a collagen-coated dish. More interestingly, GJIC of NHOsts, which has been reported to play a role in cell differentiation as well as homeostasis of cells, were not significantly different among the SAM surfaces tested. These suggest that a specific functional group on a material surface can regulate NHOst adhesion, proliferation, and differentiation via cell-functional group interaction without influencing their homeostasis.
生物材料的表面被认为会影响细胞在表面黏附后的行为，这表明生物材料的表面特性在细胞黏附、增殖和分化中起着重要作用。为了评估官能团对生物材料表面的影响，将正常人类成骨细胞（NHOsts）培养在涂有自组装单层（SAMs）的表面上，这些 SAMs 含有各种官能团，研究了 NHOsts 的黏附、增殖、分化和缝隙连接细胞间通讯（GJIC）。在具有末端甲基基团（疏水面）的 SAM 情况下，NHOst 的黏附和增殖较少。相比之下，NHOsts 在具有羟基、羧基、氨基、磷酸基和硫酸基的 SAM 上黏附良好，这些官能团相对亲水，它们的增殖和分化水平取决于官能团的类型。特别是，当它们在涂有磷酸酯或硫酸盐的 SAM 上培养时，它们的碱性磷酸酶活性和钙沉积量都比在胶原蛋白涂覆的培养皿中培养时增加了。更有趣的是，NHOsts 的 GJIC 已被报道在细胞分化以及细胞内稳态中发挥作用，在测试的 SAM 表面之间没有显著差异。这些表明，材料表面的特定官能团可以通过细胞-官能团相互作用来调节 NHOst 的黏附、增殖和分化，而不会影响它们的内稳态。
