Clinic of Small Animal Medicine, LMU University of Munich, Muenchen, Germany.
Anat Rec (Hoboken). 2010 May;293(5):770-4. doi: 10.1002/ar.21104.
The aim of the study was to elucidate expression and localization of several calcium handling proteins and the regulating vitamin D receptor (VDR) in the dog using RT-PCR and immunohistochemistry. The results of RNA expression and antibody staining were compared to ensure specificity. We found several antibodies to be useful for further studies on calcium homeostasis.
本研究旨在利用 RT-PCR 和免疫组织化学技术阐明几种钙处理蛋白和调节维生素 D 受体(VDR)在犬中的表达和定位。比较了 RNA 表达和抗体染色的结果以确保特异性。我们发现几种抗体可用于进一步研究钙稳态。