Lee Dohee, Kim Sanggu, Koo Yoonhoi, Chae Yeon, Wang Juwon, Kim Soochong, Yun Taesik, Yang Mhan-Pyo, Kang Byeong-Teck, Kim Hakhyun
Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
Laboratory of Veterinary Pathology and Platelet Signaling, College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
Front Vet Sci. 2023 Sep 21;10:1265203. doi: 10.3389/fvets.2023.1265203. eCollection 2023.
Vitamin D plays a role in anti-inflammatory processes, and the alteration of its metabolism is associated with the inflammatory processes of pancreatitis. This study was performed to evaluate the expression of the vitamin D receptor (VDR) and the two major enzymes that regulate vitamin D metabolism, 1α-hydroxylase (CYP27B1) and 24-hydroxylase (CYP24A1), in the canine pancreas and to compare their degrees of immunoreactivity between normal and inflamed pancreases. Five normal and inflamed pancreatic tissues each were obtained from six dogs. The expression of VDR, CYP24A1, and CYP27B1 were determined immunohistochemically, and the degree of immunostaining was assessed semiquantitatively. The VDR was expressed in the ducts, acini, and islets of Langerhans of normal pancreases and in the ducts and acini of inflamed ones. There was a significant difference in the immunoreactivity score for VDR in the islets of Langerhans between normal (median, 3 [interquartile range, 2-7.5] score) and inflamed pancreatic tissues (0 [0-0.5] score, = 0.03). CYP24A1 was expressed in the ducts and islets of Langerhans in both normal and inflamed pancreases, whereas CYP27B1 was expressed in the ducts and acini in only some normal and inflamed pancreatic tissues. This study showed that VDR expression decreased in inflamed pancreases and demonstrated CYP24A1 and CYP27B1 expression in the canine pancreas for the first time. These findings indicate that the pancreas could regulate the metabolism and biological activity of vitamin D and suggest that a decrease in these might be related to the pathophysiology of pancreatitis.
维生素D在抗炎过程中发挥作用，其代谢改变与胰腺炎的炎症过程相关。本研究旨在评估犬胰腺中维生素D受体（VDR）以及调节维生素D代谢的两种主要酶——1α-羟化酶（CYP27B1）和24-羟化酶（CYP24A1）的表达，并比较正常胰腺和炎症胰腺之间它们的免疫反应程度。从6只犬身上分别获取5个正常胰腺组织和5个炎症胰腺组织。采用免疫组织化学方法测定VDR、CYP24A1和CYP27B1的表达，并对免疫染色程度进行半定量评估。VDR在正常胰腺的导管、腺泡和胰岛中表达，在炎症胰腺的导管和腺泡中也有表达。正常胰腺胰岛中VDR的免疫反应评分（中位数为3分[四分位间距为2 - 7.5分]）与炎症胰腺组织（0分[0 - 0.5分]，P = 0.03）之间存在显著差异。CYP24A1在正常和炎症胰腺的导管和胰岛中均有表达，而CYP27B1仅在部分正常和炎症胰腺组织的导管和腺泡中表达。本研究首次表明炎症胰腺中VDR表达降低，并证实了犬胰腺中CYP24A1和CYP27B1的表达。这些发现表明胰腺可能调节维生素D的代谢和生物学活性，并提示其降低可能与胰腺炎的病理生理学有关。
