Laboratory of Forensic Medicine and Toxicology, Medical School, Aristotle University of Thessaloniki, Thessaloniki Greece.
J Sep Sci. 2010 Mar;33(6-7):716-27. doi: 10.1002/jssc.200900803.
Hydrophilic interaction chromatography (HILIC) is a relatively recently introduced mode of liquid-phase separations. Recently, HILIC has been used for coupling to MS in metabonomic/metabolomic studies to provide a complementary tool to the widely used reversed-phase (RP) chromatographic separations. The combination of HILIC with MS detection covers a number of polar metabolites that are typically nonretained in RPLC-mode separations and thus enlarging the number of detected analytes. This way of metabolite profiling thus provides more comprehensive metabolome coverage than using RP chromatography alone. This review describes the applications and the utility of HILIC-MS in metabolomic/metabonomic studies and highlights certain characteristic examples in the life and plant-food sciences.
亲水作用色谱（HILIC）是一种相对较新的液相分离模式。最近，HILIC 已被用于与 MS 耦合，用于代谢组学/代谢组学研究，为广泛使用的反相（RP）色谱分离提供了一种互补工具。HILIC 与 MS 检测的结合涵盖了许多在 RPLC 模式分离中通常不保留的极性代谢物，从而增加了检测到的分析物的数量。因此，这种代谢物分析的方法比单独使用 RP 色谱提供了更全面的代谢组覆盖。本文综述了 HILIC-MS 在代谢组学/代谢组学研究中的应用和实用性，并强调了生命和植物食品科学中的某些典型实例。
