Gika Helen G, Theodoridis Georgios A, Wilson Ian D
Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Cheshire, UK.
J Sep Sci. 2008 May;31(9):1598-608. doi: 10.1002/jssc.200700644.
Hydrophilic interaction chromatography (HILIC) provides a complementary technique to RP methods for the retention of polar analytes for LC-MS-based metabonomic studies. Combining the advantages of both RP and HILIC separations with the efficient and rapid separations obtained using sub-2 mum particles via the recently introduced ultra-performance LC (UPLC) enables increased coverage of the metabolites present in biological samples to be achieved. Here an HILIC-UPLC-MS method was developed to provide metabolite profiles for urine samples obtained from male Zucker rats. The resulting data were compared with results obtained for the same samples by RP-UPLC-MS and demonstrated the complementary nature of the two separations with both methods enabling discrimination between the different sample types. Interestingly sample type differentiation was based on different markers.
亲水作用色谱法（HILIC）为基于液相色谱-质谱联用的代谢组学研究中保留极性分析物提供了一种与反相（RP）方法互补的技术。将RP和HILIC分离的优势与通过最近推出的超高效液相色谱（UPLC）使用亚2微米颗粒实现的高效快速分离相结合，能够增加生物样品中代谢物的覆盖范围。本文开发了一种HILIC-UPLC-MS方法，用于提供从雄性Zucker大鼠获得的尿液样本的代谢物谱。将所得数据与通过RP-UPLC-MS对相同样本获得的结果进行比较，证明了两种分离方法的互补性，两种方法均能区分不同的样本类型。有趣的是，样本类型的区分基于不同的标志物。
