Laboratory of Neuroscience, Facultad de Psicología, Universidad de Colima, Colima, Col. 28040, México.
Glia. 2010 Jun;58(8):975-83. doi: 10.1002/glia.20979.
Adult neural stem cells (NSCs) are located in the subventricular zone (SVZ), a specialized brain niche located on the walls of the lateral ventricle. Under physiological conditions, NSCs generate a large number of young neurons and some oligodendrocytes, however the mechanisms controlling cell proliferation and migration are unclear. In vitro, epidermal growth factor (EGF) signaling has been shown to be an important mediator of cell proliferation and migration in the adult brain; however, the primary SVZ progenitors that respond to EGF are not well known. In this study, we isolated SVZ type-B astrocytes and cultured them under different EGF concentrations. We found a dose-dependent effect of EGF on proliferation rates and migration of SVZ type-B astrocytes. We found that GFAP+ type-B astrocytes gave rise to highly migratory and proliferating cells that expressed Olig2 and NG2. After EGF withdrawal, a significant number of EGF-stimulated cells differentiated into S100beta+/O4+ oligodendrocytes. This study provides new insights about the production of oligodendrocytes derived from the astrocyte NSCs residing in the adult SVZ. To be able to manipulate the endogenous adult progenitors, it is crucial to identify and isolate the responding primary precursors and determine the extracellular signals that regulate their cell division, migration, and fate.
成年神经干细胞（NSCs）位于侧脑室壁上的一个特殊的脑区——侧脑室下区（SVZ）。在生理条件下，NSCs 会产生大量的年轻神经元和一些少突胶质细胞，但控制细胞增殖和迁移的机制尚不清楚。在体外，表皮生长因子（EGF）信号已被证明是成年大脑中细胞增殖和迁移的重要介质；然而，对 EGF 有反应的主要 SVZ 祖细胞尚不清楚。在这项研究中，我们分离了 SVZ 型星形胶质细胞，并在不同的 EGF 浓度下培养它们。我们发现 EGF 对 SVZ 型星形胶质细胞的增殖率和迁移有剂量依赖性的影响。我们发现，GFAP+型星形胶质细胞产生了高度迁移和增殖的细胞，这些细胞表达 Olig2 和 NG2。EGF 撤出后，大量的 EGF 刺激细胞分化为 S100beta+/O4+少突胶质细胞。这项研究为 SVZ 中星形胶质细胞来源的少突胶质细胞的产生提供了新的见解。为了能够操纵内源性的成年祖细胞，识别和分离反应性的原始前体细胞并确定调节它们细胞分裂、迁移和命运的细胞外信号是至关重要的。
