Laboratory for Plant Molecular Biology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade, Serbia.
J Agric Food Chem. 2010 Mar 24;58(6):3488-94. doi: 10.1021/jf904483a.
The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu(2+) and Cd(2+) treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.
研究了荞麦金属硫蛋白 3（FeMT3）在金属胁迫下的体内保护作用及其启动子对金属离子的响应。检测到产 FeMT3 的大肠杆菌和 cup1(Delta)酵母细胞对重金属的耐受性增加。在瞬时表达 FeMT3 的 Nicotiana debneyii 叶片中也证明了荞麦 MT3 在 Cd 和 Cu 胁迫下的防御能力。与植物螯合肽不同，在重金属胁迫下，FeMT3 的细胞质定位没有改变。对相应启动子区域的功能分析表明，Cu(2+)和 Cd(2+)处理后具有极高的诱导性。FeMT3 蛋白在体内的防御能力得到证实，其启动子在重金属暴露下的高度响应性使该基因成为生物技术应用的合适候选。
