Milisavljević Mira Dj, Konstantinović Miroslav M, Brkljacić Jelena M, Maksimović Vesna R
Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia and Montenegro.
J Agric Food Chem. 2005 Mar 23;53(6):2076-80. doi: 10.1021/jf048330g.
Using the modified rapid amplification of cDNA ends (5'-RACE) approach, a fragment containing the 955 bp long 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced, and the sequence was analyzed by computer prediction of cis-regulatory elements possibly involved in tissue-specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research but also as a useful tool for biotechnological application.
利用改良的cDNA末端快速扩增（5'-RACE）方法，从荞麦基因组DNA中扩增出一个包含955 bp长的荞麦贮藏球蛋白基因（FeLEG1）5'-调控区的片段。对整个片段进行测序，并通过计算机预测可能参与组织特异性和发育调控的种子贮藏蛋白基因表达的顺式调控元件来分析该序列。所获得的启动子不仅可能对基础研究有意义，而且作为生物技术应用的有用工具也很有价值。
