Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Org Lett. 2010 Apr 2;12(7):1608-11. doi: 10.1021/ol100338v.
The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
本文描述了一种新型荧光脂质 II 基底物用于转糖苷酶（TGases）的制备。这种底物具有特征性的结构特征，包括较短的脂质链、脂质链末端的荧光团标记而不是肽链上的标记，以及不标记放射性原子。这种荧光底物可方便地用于 TGase 活性测定，以表征 TGase，也可用于评估 TGase 抑制剂的活性。
