急性心肌梗死大鼠心肌中 microRNAs 的表达改变。
Altered expression of microRNAs in the myocardium of rats with acute myocardial infarction.
机构信息
Department of Cardiology, Peking University Third Hospital, Beijing, China.
出版信息
BMC Cardiovasc Disord. 2010 Mar 1;10:11. doi: 10.1186/1471-2261-10-11.
BACKGROUND
MicroRNAs(miRNAs) are important cellular components and their dysfunction is associated with various diseases. Acute myocardial infarction (AMI) is one of the most serious cardiovascular diseases. Although several miRNAs are reported to be associated with AMI, more novel miRNAs are needed to further investigate and improve certainty
METHODS
We applied a well-established acute myocardial infarction rat model and performed miRNAs microarray experiments upon the myocardium tissue of rats with AMI and under sham control. We identified the differentially expressed miRNAs and analyzed the function of miRNA targets, transcription factors, and host genes based on bioinformatics.
RESULTS
As a result, the levels of expression of seventeen miRNAs significantly deregulated, of which four miRNAs were further validated by qRT-PCR. In addition, we observed that the transcription factors, targets, and host genes of these deregulated miRNAs are enriched in cardiovascular-related functions.
CONCLUSION
We found that the miRNAs expression level altered in rats with AMI and differentially expressed miRNAs may be novel biomarkers of AMI.
背景
MicroRNAs(miRNAs) 是重要的细胞成分，其功能障碍与各种疾病有关。急性心肌梗死 (AMI) 是最严重的心血管疾病之一。尽管有报道称几种 miRNAs 与 AMI 有关，但需要更多新的 miRNAs 来进一步研究和提高确定性。
方法
我们应用了一种成熟的急性心肌梗死大鼠模型，并对 AMI 大鼠和假手术对照组的心肌组织进行了 miRNAs 微阵列实验。我们基于生物信息学分析鉴定了差异表达的 miRNAs，并分析了 miRNA 靶标、转录因子和宿主基因的功能。
结果
结果，十七个 miRNAs 的表达水平显著失调，其中四个 miRNAs 通过 qRT-PCR 进一步验证。此外，我们观察到这些失调 miRNAs 的转录因子、靶标和宿主基因富集在心血管相关功能中。
结论
我们发现 AMI 大鼠中 miRNAs 的表达水平发生改变，差异表达的 miRNAs 可能是 AMI 的新型生物标志物。
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