Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi-22 1005, India.
Biochem Biophys Res Commun. 2010 Mar 26;394(1):163-9. doi: 10.1016/j.bbrc.2010.02.135. Epub 2010 Feb 24.
This study presents first hand data on the cloning and heterologous expression of Anabaena PCC 7120 all3940 (a dps family gene) in combating nutrients limitation and multiple abiotic stresses. The Escherichia coli transformed with pGEX-5X-2-all3940 construct when subjected to iron, carbon, nitrogen, phosphorus limitation and carbofuron, copper, UV-B, heat, salt and cadmium stress registered significant increase in growth over the cells transformed with empty vector under iron (0%), carbon (0.05%), nitrogen (3.7 mM) and phosphorus (2mM) limitation and carbofuron (0.025 mg ml(-1)), CuCl(2) (1 mM), UV-B (10 min), heat (47 degrees C), NaCl (6% w/v) and CdCl(2) (4mM) stress. Enhanced expression of all3940 gene measured by semi-quantitative RT-PCR at different time points under above mentioned treatments clearly demonstrates its role in tolerance against aforesaid abiotic stresses. This study opens the gate for developing transgenic cyanobacteria capable of growing successfully under above mentioned stresses.
本研究提供了关于在应对营养限制和多种非生物胁迫时克隆和异源表达鱼腥藻 PCC 7120 all3940（一种 dps 家族基因）的第一手数据。将 pGEX-5X-2-all3940 构建体转化的大肠杆菌在受到铁、碳、氮、磷限制以及carbofuron、铜、UV-B、热、盐和镉胁迫时，与空载体转化的细胞相比，生长显著增加在铁（0%）、碳（0.05%）、氮（3.7 mM）和磷（2mM）限制以及carbofuron（0.025 mg ml(-1)）、CuCl(2)（1 mM）、UV-B（10 min）、热（47 摄氏度）、NaCl（6% w/v）和 CdCl(2)（4mM）胁迫下。在上述处理的不同时间点通过半定量 RT-PCR 测量的 all3940 基因的增强表达清楚地表明了它在耐受上述非生物胁迫中的作用。这项研究为开发能够在上述胁迫下成功生长的转基因蓝细菌开辟了道路。
