Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Gene. 2019 Feb 15;685:230-241. doi: 10.1016/j.gene.2018.11.038. Epub 2018 Nov 15.
In- silico and functional genomics approaches have been used to determine cellular functions of two hypothetical proteins All1122 and Alr0750 of Anabaena sp. PCC 7120. Motif analysis and multiple sequence alignment predicted them as typical α/β ATP binding universal stress family protein-A (UspA) with G-(2×)-G-(9×)-G(S/T) as conserved motif. qRT-PCR data under UV-B, NaCl, heat, As, CdCl mannitol and methyl viologen registered approximately 1.4 to 4.3 fold induction of all1122 and alr0750 thus confirming their multiple abiotic stress tolerance potential. The recombinant E. coli (BL21) cells harboring All1122 and Alr0750 showed 12-41% and 23-41% better growth respectively over wild type control under said abiotic stresses thus revalidating their stress coping ability. Functional complementation on heterologous expression in UspA mutant E. coli strain LN29MG1655 (ΔuspA::Kan) attested their UspA family membership. This study tempted us to suggest that recombinant Anabaena PCC 7120 over expressing all1122 and alr0750 might contribute to the nitrogen economy in paddy fields experiencing array of abiotic stresses including drought and nutrient limitation.
已经使用计算机模拟和功能基因组学方法来确定 Anabaena sp. PCC 7120 中的两个假设蛋白 All1122 和 Alr0750 的细胞功能。基序分析和多序列比对预测它们为典型的α/β ATP 结合通用应激家族蛋白-A (UspA)，具有 G-(2×)-G-(9×)-G(S/T) 作为保守基序。在 UV-B、NaCl、热、As、CdCl 甘露醇和甲基紫精下的 qRT-PCR 数据记录了 all1122 和 alr0750 的约 1.4 到 4.3 倍诱导，从而证实了它们的多种非生物胁迫耐受性潜力。在上述非生物胁迫下，携带 All1122 和 Alr0750 的重组大肠杆菌 (BL21) 细胞的生长分别比野生型对照提高了 12-41%和 23-41%，从而再次验证了它们的应激应对能力。在 UspA 突变大肠杆菌菌株 LN29MG1655（ΔuspA::Kan）中的异源表达的功能互补证实了它们属于 UspA 家族。这项研究促使我们提出，过表达 all1122 和 alr0750 的重组 Anabaena PCC 7120 可能有助于在经历包括干旱和营养限制在内的多种非生物胁迫的稻田中的氮素经济。
