利用 DNA 疫苗对分枝杆菌 DNA 结合蛋白 1（MDP1）上的小鼠 T 细胞表位进行表征。
Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination.
机构信息
3rd Department of Internal Medicine, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu, Japan.
出版信息
Vaccine. 2010 Feb 23;28(8):2020-5. doi: 10.1016/j.vaccine.2009.10.062.
Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-gamma production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection.
分枝杆菌 DNA 结合蛋白 1（MDP1）是分枝杆菌中的主要蛋白抗原，可诱导小鼠对结核分枝杆菌感染产生保护性免疫。在这项研究中，我们通过分枝杆菌 DNA 免疫小鼠来确定 MDP1 上的小鼠 T 细胞表位。我们分析了 MDP1 DNA 免疫脾细胞对覆盖 MDP1 蛋白的 20 个重叠肽的干扰素-γ产生。我们在三个近交系小鼠和一个 C57BL/6 小鼠中鉴定了几个 CD4+T 细胞表位和一个 CD8+T 细胞表位。这些 T 细胞表位可用于分析 MDP1 特异性 T 细胞在保护性免疫中的作用，以及未来针对结核分枝杆菌感染的疫苗设计。
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