Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan.
Vaccine. 2010 Feb 23;28(8):2026-31. doi: 10.1016/j.vaccine.2009.10.063.
We identified a novel HLA-DR4-restricted CD4+ T-cell epitope on a secreted antigen of Mycobacterium tuberculosis, MPT51, in 004149-MM HLA-DR4-transgenic mice which express HLA-DRB1*0401, but not murine MHC class II molecules. The mice were immunized with plasmid DNA encoding MPT51 using gene gun and interferon (IFN)-gamma production from the immune splenocytes was analyzed. In response to overlapping synthetic peptides covering the mature MPT51 sequence, only one peptide, p191-210, stimulated the splenocytes to produce IFN-gamma. Further analysis using flow cytometry and computer-assisted algorithm, ProPred, narrowed down the region of CD4+ T-cell epitope to p191-202. The CD4+ T-cell epitope would be feasible for vaccine design against tuberculosis as well as for analysis of MPT51-specific T-cells in M. tuberculosis infection.
我们在 004149-MM HLA-DR4 转基因小鼠中鉴定出一种新型结核分枝杆菌分泌抗原 MPT51 的 HLA-DR4 限制性 CD4+ T 细胞表位，该小鼠表达 HLA-DRB1*0401，但不表达鼠 MHC 类 II 分子。用基因枪将编码 MPT51 的质粒 DNA 免疫小鼠，分析免疫脾细胞产生的干扰素 (IFN)-γ。针对覆盖成熟 MPT51 序列的重叠合成肽，只有一个肽，p191-210，刺激脾细胞产生 IFN-γ。使用流式细胞术和计算机辅助算法 ProPred 进一步分析，将 CD4+ T 细胞表位的区域缩小到 p191-202。该 CD4+ T 细胞表位可用于结核病疫苗设计以及结核分枝杆菌感染中 MPT51 特异性 T 细胞的分析。
