School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, 13 Yanta Road, Xi'an 710055, China.
Chemosphere. 2010 Mar;79(2):180-5. doi: 10.1016/j.chemosphere.2010.02.007. Epub 2010 Feb 25.
A lab-scale sequencing batch reactor was operated in batch mode to treat ammonium-rich synthetic wastewater, and minor N loss (about 9%) was observed without seeding anaerobic ammonium oxidation bacteria (AnAOB). Around 4 wk after the dissolved oxygen concentration was decreased (from 0.9+/-0.1 to 0.4+/-0.1 mg L(-1)) and the hydraulic retention time was elevated (from 11.0 to 14.7 h), 80% of total nitrogen removal efficiency and 0.49 kg N m(-3) d(-1) of maximum nitrogen removal capacity were obtained. During a single cycle, a peak of NO2(-) concentration was observed at 60 min, and the pH first increased from 8.02 to 8.17 and then returned to 8.04. Fluorescence in situ hybridization analysis revealed that the aerobic ammonium-oxidizing genus Nitrosomonas dominated the community, while AnAOB and very small amounts of Nitrospira spp. were also detected. The ratio of nitrate produced to ammonia consumed (0.10+/-0.02) was lower than that achieved with the completely autotrophic nitrogen removal over nitrite process. This study revealed that nitritation, Anammox and autotrophic denitrification were responsible for the nitrogen removal. The nitrate production was caused by the co-action of nitrite-oxidizing bacteria and AnAOB.
采用实验室规模的序批式反应器在分批模式下处理富含氨氮的合成废水，在没有接种厌氧氨氧化菌（AnAOB）的情况下观察到少量的氮损失（约 9%）。在溶解氧浓度降低（从 0.9+/-0.1 降至 0.4+/-0.1 mg/L）和水力停留时间延长（从 11.0 升至 14.7 h）约 4 周后，总氮去除效率达到 80%，最大氮去除能力达到 0.49 kg N m(-3) d(-1)。在单个循环中，观察到 60 分钟时出现 NO2(-)浓度峰值，pH 值先从 8.02 增加到 8.17，然后回到 8.04。荧光原位杂交分析表明，好氧氨氧化菌属 Nitrosomonas 主导了群落，同时也检测到了少量的 AnAOB 和 Nitrospira spp.。产生的硝酸盐与消耗的氨的比例（0.10+/-0.02）低于完全自养亚硝酸盐途径脱氮工艺所达到的比例。本研究表明，硝化、厌氧氨氧化和自养反硝化是氮去除的原因。硝酸盐的产生是由亚硝酸盐氧化菌和 AnAOB 的共同作用引起的。
