Lee S H
Department of Haematology, Monash Medical Centre, Prince Henry's Hospital, Melbourne, Victoria, Australia.
Blood Cells. 1991;17(1):45-54; discussion 54-8.
Bone marrow macrophages have been isolated for phenotypic analysis by their ability to bind erythroblasts in erythroid clusters. In situ, when labeled for CD68 antigen, they are seen to form an arborizing network uniformly distributed throughout the hemopoietic marrow. Marrow macrophages isolated within erythroid clusters are acid phosphatase and alpha-naphthol butyrate esterase positive. Immunophenotypically, they are highly reactive for CD4, phagocytic receptors FcRI, II, and III, for HLA-Dr and CD31, as well as for the integrins CD11a, CD11c, and CD18, but negative for CD35 and transferrin receptor epitopes. Comparison of their phenotype with blood monocytes and cultured macrophages reveals significant differences, which indicate that marrow macrophages are specialized, differentiated mononuclear phagocytes that selectively associate with developing erythroblasts.
骨髓巨噬细胞已通过其在红系簇中结合成红细胞的能力被分离出来进行表型分析。在原位，当用CD68抗原标记时，可以看到它们形成一个分支状网络，均匀分布于整个造血骨髓中。在红系簇内分离出的骨髓巨噬细胞酸性磷酸酶和α-萘酚丁酸酯酶呈阳性。免疫表型上，它们对CD4、吞噬受体FcRI、II和III、HLA-Dr和CD31以及整合素CD11a、CD11c和CD18具有高度反应性，但对CD35和转铁蛋白受体表位呈阴性。将它们的表型与血液单核细胞和培养的巨噬细胞进行比较，发现存在显著差异，这表明骨髓巨噬细胞是专门化的、分化的单核吞噬细胞，可选择性地与发育中的成红细胞结合。
