造血簇中人类骨髓基质巨噬细胞的分离及免疫细胞化学特性分析
Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters.
作者信息
Lee S H, Crocker P R, Westaby S, Key N, Mason D Y, Gordon S, Weatherall D J
机构信息
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
出版信息
J Exp Med. 1988 Sep 1;168(3):1193-8. doi: 10.1084/jem.168.3.1193.
Abstract
Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD32+, FcRI+, HLA-DR+, and CD35-, transferrin receptor-negative, and CD11b (weak). They express endocytic receptor antigens, but show significant differences in myeloid antigen expression compared with freshly harvested or cultured monocytes. Human stromal M phi are therefore specialized mature marrow M phi that are accessible for further investigations in infectious, storage, or hemopoietic disorders.
摘要
基质巨噬细胞（M phi）已在原位定位，并从人骨髓的红系簇中分离出来。基质M phi在广泛分布于整个骨髓间质的广泛网络中形成树枝状，并表达CD4 +、CD11a +、CD11c +、CD13 +、CD14 +、CD16 +、CD18 +、CD31 +、CD32 +、FcRI +、HLA - DR +和CD35 -、转铁蛋白受体阴性以及CD11b（弱阳性）的表型。它们表达内吞受体抗原，但与新鲜收获或培养的单核细胞相比，在髓系抗原表达上存在显著差异。因此，人基质M phi是特殊的成熟骨髓M phi，可用于对感染性、贮积性或造血性疾病进行进一步研究。
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