Barr Torin, McNamara Audrey J A, Sándor George K B, Clokie Cameron M L, Peel Sean A F
Oral and Maxillofacial Surgery and Anesthesia, University of Toronto, Toronto, Ontario, Canada.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Apr;109(4):531-40. doi: 10.1016/j.tripleo.2009.10.027. Epub 2010 Feb 26.
Recent research has focused on application of growth factors such as bone morphogenetic proteins (BMPs) as alternatives to autogenous bone grafting. Two bone graft substitute bioimplants containing recombinant human BMPs (rhBMPs), Infuse (rhBMP-2) and OP-1 (rhBMP-7), are approved for human application but have never been compared side by side. The aim of this study was to provide a direct comparison of the osteoinductive activity of the 2 commercially available and approved rhBMP-containing bioimplants in their clinically available forms.
The activity of rhBMP-2 and -7 in solution were compared in vitro using the C2C12 cell-based assay. The activity of Infuse and OP-1 bioimplants containing 52.5 microg of rhBMP-2 or rhBMP-7, respectively, were compared in vivo using a mouse muscle pouch assay and analyzed by microscopic CT (microCT) and histology.
The in vitro results showed that rhBMP-2 stimulated greater alkaline phosphatase production than rhBMP-7 over various time points and concentrations. The in vivo results showed that OP-1 induced greater bone volume than Infuse. Both implants induced bone of equivalent quality based on microCT and histologic evaluation.
In their clinically available forms, the rhBMP-7-containing OP-1 induced greater bone volume than the rhBMP-2-containing Infuse in the mouse muscle pouch model.
近期研究聚焦于骨形态发生蛋白（BMPs）等生长因子作为自体骨移植替代物的应用。两种含重组人BMPs（rhBMPs）的骨移植替代生物植入物，即Infuse（rhBMP - 2）和OP - 1（rhBMP - 7）已获批准用于人体，但从未进行过直接比较。本研究的目的是对这两种市售且已获批准的含rhBMP生物植入物的临床可用形式的骨诱导活性进行直接比较。
使用基于C2C12细胞的检测方法在体外比较rhBMP - 2和 - 7在溶液中的活性。分别使用含52.5微克rhBMP - 2或rhBMP - 7的Infuse和OP - 1生物植入物，通过小鼠肌肉袋检测在体内进行比较，并通过显微CT（microCT）和组织学分析。
体外结果显示，在不同时间点和浓度下，rhBMP - 2比rhBMP - 7刺激产生更多的碱性磷酸酶。体内结果显示，OP - 1诱导产生的骨体积比Infuse更大。基于microCT和组织学评估，两种植入物诱导产生的骨质量相当。
在小鼠肌肉袋模型中，临床可用形式的含rhBMP - 7的OP - 1诱导产生的骨体积比含rhBMP - 2的Infuse更大。
