BIA Separations, d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.
J Virol Methods. 2010 Jun;166(1-2):60-4. doi: 10.1016/j.jviromet.2010.02.020. Epub 2010 Feb 25.
Bacteriophages (phages) are known to be useful in many fields from medicine to agriculture, and for a broad range of applications, including phage therapy and phage display. For some applications, especially in medicine, high purity and viability of phages are required. Methacrylate monoliths (Convective Interaction Media [CIM] monolithic columns), designed for purification of bionanoparticles, were applied for the purification of Staphylococcus aureus phages VDX-10 from bacterial lysate. With a single step purification method, more than 99% of host cell DNA and more than 90% of proteins were removed, with 60% recovery of viable phages. Comparable results were obtained when the purification method was scaled-up from a CIM monolithic disk to a larger CIM monolithic column. Additionally, the dynamic binding capacity of a methacrylate monolith column for S. aureus phages VDX-10 was determined.
噬菌体（phages）在医学到农业等众多领域都有应用，具有广泛的用途，包括噬菌体治疗和噬菌体展示。对于某些应用，特别是在医学方面，需要噬菌体具有高纯度和高活力。用于生物纳米粒子纯化的甲基丙烯酸酯整体柱（Convective Interaction Media [CIM] 整体柱）被应用于从细菌裂解物中纯化金黄色葡萄球菌噬菌体 VDX-10。通过单一的纯化步骤，去除了超过 99%的宿主细胞 DNA 和超过 90%的蛋白质，同时保持 60%的噬菌体活力。当从 CIM 整体盘放大到更大的 CIM 整体柱时，获得了相当的结果。此外，还确定了用于金黄色葡萄球菌噬菌体 VDX-10 的甲基丙烯酸酯整体柱的动态结合容量。
