FIA Laboratory, Analytical Chemistry Section, INQUISUR (UNS-CONICET), Av. Alem 1253, B8000CPB Bahía Blanca, Buenos Aires, Argentina.
Talanta. 2010 Apr 15;81(1-2):116-9. doi: 10.1016/j.talanta.2009.11.045. Epub 2009 Nov 24.
The advantages of the flow-batch methodology were exploited to implement a simple system with nephelometric detection for the determination of monosodium glutamate (MSG) in food samples. The method is based on the inhibitory effect of the MSG over the crystallization of L-lysine in an isopropanol/acetone mixture. The calibration curve was prepared on-line. The method was linear over the range of 2.8 x 10(-3) to 1.1 x 10(-2)gL(-1) and a detection limit of 9.7 x 10(-5)gL(-1) was achieved. It was successfully applied to determine the MSG concentration in food samples, without a previous treatment. A recovery study was carried out on real samples and the percentages were between 98 and 106%.
利用流批方法的优势，实现了一种带有比浊检测的简单系统，用于测定食品样品中的谷氨酸单钠（MSG）。该方法基于 MSG 对 L-赖氨酸在异丙醇/丙酮混合物中结晶的抑制作用。在线制备校准曲线。该方法在 2.8 x 10(-3) 至 1.1 x 10(-2) gL(-1) 的范围内呈线性，检测限为 9.7 x 10(-5) gL(-1)。它成功地应用于测定食品样品中的 MSG 浓度，无需事先处理。对实际样品进行了回收率研究，回收率在 98%至 106%之间。
