Chemistry Department, M.V. Lomonosov Moscow State University, Vorob'evy Hills d. 1, str. 3, 119991 GSP-1 Moscow, Russia.
Talanta. 2010 Apr 15;81(1-2):377-84. doi: 10.1016/j.talanta.2009.12.012. Epub 2009 Dec 21.
As a primary aim, several approaches to photothermal (thermal-lens) and optoacoustic determination of exogenous pyrogens (lipopolysaccharides) based on photometric procedures for their determination at the microgram level and below are compared. The limits of detection of lipopolysaccharides by thermal-lens spectrometry and optoacoustic spectroscopy are at a level of 2-100 ng/mL, and the conditions of optoacoustic and photothermal procedures are the same. Optoacoustic spectroscopy is advantageous in determining suspensions, while thermal lensing is superior in determining lipopolysaccharides from homogenous aqueous solutions. As a secondary aim, photometric procedures for lipopolysaccharides by the formation of their ion pairs with several dyes and by the reaction of 2-keto-3-deoxyoctulosonic acid as a part of a lipopolysaccharide molecule with thiobarbituric acid are optimized. In the case of the 2-keto-3-deoxyoctulosonic acid reaction, the sampling stage time is decreased twofold, and the possibility of substitution of the toxic metaarsenite for sulfite with better sensitivity is shown.
作为主要目标，比较了几种基于光度法测定外源性热原（脂多糖）的光热（热透镜）和光声测定方法，这些方法可用于测定微克级及以下水平的脂多糖。热透镜光谱法和光声光谱法测定脂多糖的检测限为 2-100ng/mL，光声和光热程序的条件相同。光声光谱法在测定悬浮液方面具有优势，而热透镜法在测定均相水溶液中的脂多糖方面更优越。作为次要目标，优化了几种染料与脂多糖形成离子对以及脂多糖分子中 2-酮-3-脱氧辛糖酸与硫代巴比妥酸反应形成其离子对的光度法测定脂多糖的方法。在后一种 2-酮-3-脱氧辛糖酸反应中，采样阶段时间缩短了两倍，并且显示出用毒性较小的偏亚砷酸盐替代亚硫酸盐的可能性，同时具有更好的灵敏度。
