用高效阴离子交换色谱法（HPAEC）测定2-酮-3-脱氧辛糖酸（KDO）：KDO稳定性及最佳水解条件研究
Determination of 2-keto-3-deoxyoctulosonic acid (KDO) with high-performance anion-exchange chromatography (HPAEC): survey of stability of KDO and optimal hydrolytic conditions.
作者信息
Kiang J, Szu S C, Wang L X, Tang M, Lee Y C
机构信息
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
出版信息
Anal Biochem. 1997 Feb 1;245(1):97-101. doi: 10.1006/abio.1996.9951.
PMID:9025974
Abstract
A new method for 2-keto-3-deoxy-octulosonic acid (KDO) determination using high-performance anion-exchange chromatography was developed. KDO is well separated from Neu5Ac, and the response was linear from 20 pmol to 5 nmol. The method was used to examine the stability of KDO under various hydrolytic, conditions and to survey optimal hydrolytic conditions for release of KDO.
摘要
开发了一种使用高效阴离子交换色谱法测定2-酮基-3-脱氧辛糖酸（KDO）的新方法。KDO与Neu5Ac能很好地分离，响应在20皮摩尔至5纳摩尔范围内呈线性。该方法用于检测KDO在各种水解条件下的稳定性，并探寻释放KDO的最佳水解条件。
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